细粒棘球蚴慢性感染阶段小鼠血清中炎症因子的变化及意义  

Changes and significance of inflammatory cytokines in sera of mice chronically infected with larvae of Echinococcus granulosus

作　　者：潘伟[1] 李向阳[1] 孙芬芬 郝文婷[1] 秦苏萍[1] 颜超[1] 汤仁仙[1] 沈玉娟[3] 郑葵阳[1] 

机构地区：[1]徐州医科大学病原生物学与免疫学教研室,感染与免疫实验室,徐州221004 [2]徐州医科大学形态学实验教学中心 [3]中国疾病预防控制中心寄生虫病预防控制所,卫生部寄生虫病原与媒介生物学重点实验室,科技部国家级热带病国际联合研究中心,世界卫生组织热带病合作中心

出　　处：《中国血吸虫病防治杂志》2016年第5期527-529,535,共4页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81501762;81371842);中国博士后基金(2015M581864);江苏省教育厅高校自然科学研究基金(15KJB310025);江苏省博士后科研资助计划(1501061A);江苏省高校优势学科建设工程资助项目(PAPD);徐州医学院优秀人才启动基金(D2015004)

摘　　要：目的研究细粒棘球蚴慢性感染阶段小鼠血清中细胞因子水平的变化,探索机体对抗寄生虫感染的免疫应答机制。方法从病羊内脏分离细粒棘球绦虫原头节,注射入BALB/c小鼠腹腔（2 000个原头节/只）,建立细粒棘球蚴感染小鼠模型,对照组小鼠腹腔注射等体积PBS。于感染5个月后,采集对照及感染组小鼠血清,采用流式液相多重蛋白定量技术检测血清中多种细胞因子水平并分析。结果感染组小鼠腹腔、肝脏、肺脏等部位均出现多个单一性包囊;其血清中IL-17A、IL-6、IFN-γ、MCP-1、IL-12P70、TNF-α等炎症因子水平均显著高于对照组（t=2.713～9.255,P均〈0.05）;而抑炎因子IL-10水平亦显著升高（t=3.936,P〈0.001）。结论细粒棘球蚴慢性感染阶段小鼠体内炎症因子水平较高,有助于抑制虫体生长。Objective To investigate the levels of cytokines in the sera of mice chronically infected with the larvae of Echinococcus granulosus,and explore the mechanisms of immune regulation against parasite infection. Methods The protoscoleces were isolated from the livers and lungs of sheep infected with E. granulosus,and then inoculated intraperitoneally to BALB/c mice（2 000 for each mouse）,to establish the mouse model of E. granulosus infection. The mice in the control group were injected intraperitoneally with an equal volume of PBS. The sera of both control and infected mice were collected to test the levels of multiple cytokines by using the Cytometric Bead Array（CBA）5 months post-infection. Results In contrast to the control group,the multiple cysts were found in the abdominal cavity,livers and lungs of the infected mice. Moreover,the levels of inflammatory cytokines such as IL-17 A,IL-6,IFN-γ,MCP-1,IL-12P70 and TNF-α in the sera of the infected mice were significantly higher than those in the control group（t = 2.713-9.255,all P 〈0.05）while the levels of anti-inflammatory cytokine IL-10 were elevated post-infection（t = 3.936,P 〈0.001）. Conclusion Higher inflammatory cytokines in the mice chronically infected with the larvae of E. granulosus,may benefit for the limitation of parasite growth.

关 键 词：细粒棘球绦虫 原头节 炎症因子 细胞因子微球检测 

分 类 号：R392.8[医药卫生—免疫学]