呈色机制不同的桃叶片花色素苷积累及合成相关基因表达的季节性差异  

Seasonal difference in the expression pattern of genes related to anthocyanin accumulation and biosynthesis in leaves of peach with different coloration modes

作　　者：王小青[1] 韩键[1] 文杨[1] 姜卫兵[1] 房经贵[1] 张斌斌[2] 马瑞娟[2] 

机构地区：[1]南京农业大学园艺学院,江苏南京210095 [2]江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014

出　　处：《南京农业大学学报》2016年第6期924-931,共8页Journal of Nanjing Agricultural University

基　　金：现代农业产业技术体系建设专项资金(CARS-31)

摘　　要：[目的]红叶桃叶片夏季呈现高温"返青"现象,而早熟桃夏季果实采收后叶片逐渐变红,2种桃叶片呈色规律相反。测定其花色素苷生物合成相关结构基因和转录因子表达特性,分析与叶片呈色之间的关系。[方法]以红叶桃品种‘筑波5号’和‘洛格红叶’、早熟桃品种‘早美’和‘春蕾’以及绿叶桃品种‘京蜜’为试材,分别在春、夏、秋3个季节测定其叶片花色素苷含量、叶片色差以及与叶片花色素苷生物合成相关的结构基因(CHS、CHI、F3H、F3'H、DFR、LDOX、UFGT)和调节基因(MYB10、b HLH3、WD40、MYBPA1、MYB15、MYB16、MYB111、MYB123)的时空表达。[结果]2个红叶桃品种花色素苷含量在夏季下降,秋季回升;2个早熟桃品种夏季叶片逐渐变红,花色素苷含量一直增加;而绿叶桃叶片一直处于绿色,花色素苷含量基本保持稳定。结构基因中CHS、CHI、F3'H、DFR、LDOX、UFGT和调节基因中MYB10、MYBPA1、MYB16、MYB111、WD40、b HLH3的表达水平与桃叶片中花色素苷含量呈正相关;而MYB15和MYB123基因的表达水平与桃叶片中花色素苷含量呈负相关。[结论]6个结构基因和6个调节基因表达水平与红叶桃、早熟桃花色素苷积累关系密切,表明其在调控桃叶片着色过程中起重要作用。[ Objectives] Upon the high temperature stress in summer,the leaf coloration of red leaf peach will go back to green from red, while the leaf coloration of early maturity peach gradually turns to red from green after fruit harvest. We thus determined the expression pattern of structural genes and transcription factors related to anthocyanins biosynthesis in these cultivars. Furthermore, we analyzed the relationship between the gene expression pattern with the leaf coloration to explore the underlying mechanisms. [ Methods] The experimental materials in this study include two cultivars of red leaf peach （ ＇ Tsukuba-5＇ ＇ Rutgers Redleaf＇ ）, two cultivars of early maturity peach（ ＇ Zaomei＇ ＇ Chunlei＇ ） and one cultivar of green leaf peach（ ＇ Jingmi＇ ）. During the growth seasons of peach of spring, summer and winter, the following items have been measured and compared,including the anthocyanins contents in the leaves, the color changes and the expression of the anthocyanins structural genes （ CHS, CHI, F3H, F3＇H, DFR, LDOX, UFGT） and regulatory genes（ MYBIO ,bHLH3, WD40,MYBPA1 ,MYB15 ,MYB16,MYB111 ,MYB123）. [ Results ] Anthocyanin contents of red leaf peach was down-regulated in summer, and then up-regulated in autumn. Early maturity peach leaves gradually turned to red, and the anthocyanin contents also increased in summer. Green peach leaf was keeping the color of green, and the anthocyanin contents were stable in it. Moreover,we observed that the expression of anthocyanin structural genes（ CHS, CHI, F3＇H, DFR, LDOX, UFGT） and regulatory genes（MYB10,MYBPA1 ,MYB16,MYB11l, WD40,MYB123）were positively correlated with the anthocyanin contents, while the expression of MYB15 and MYB123 were negatively correlated with the leaf anthocyanin contents in the leaves of red leaf peach and early maturing peach. [ Conclusions ] The expression patterns of six anthocyanin structural genes and six regulatory genes had close relation with the accumulation of anthocyanin in peach. We

关 键 词：桃叶 呈色 花色素苷 结构基因 转录因子 

分 类 号：S662.1[农业科学—果树学]