基于环介导等温扩增技术检测黄淮地区大豆主栽品种种子携带的病原菌  

Detection of soybean seed-borne pathogens in Huang-huai area using LAMP assays

作　　者：曾丹丹[1] 张海峰[1] 田擎[1] 许苗[1] 王源超[1] 郑小波[1] 

机构地区：[1]南京农业大学植物保护学院,江苏南京210095

出　　处：《南京农业大学学报》2016年第6期947-953,共7页Journal of Nanjing Agricultural University

基　　金：国家公益性行业(农业)科研专项(201303018);国家863计划项目(2012AA101501)

摘　　要：[目的]通过对黄淮地区大豆种子带菌的快速检测,了解该地区大豆种子携带病原菌的情况。[方法]采用环介导等温扩增技术(loop-mediated isothermal amplification,LAMP)检测黄淮地区29个大豆主栽品种种子上平头炭疽菌、胶孢炭疽菌、尖镰孢菌、木贼镰孢菌、层出镰孢菌、黄色镰孢菌、禾谷镰孢菌、茄腐镰孢菌、大豆拟茎点种腐病菌、立枯丝核菌、大豆炭腐病菌、冬青丽赤壳菌、大豆疫霉病菌等大豆主要病原菌的携带情况。[结果]供试的29个品种中有20个品种的种子样本检出携带上述病原菌,分别携带所检测的13种病原菌中的7种,检出率由高到低依次为平头炭疽菌、大豆拟茎点种腐病菌、木贼镰孢菌、黄色镰孢菌、尖镰孢菌、胶孢炭疽菌和层出镰孢菌,但不同大豆品种种子携带病原菌的种类及数量有较大差异,其中品种‘漯39001’检出的病原菌多达6种。[结论]本研究为大豆种子带菌检测提供了新的方法,并揭示了黄淮地区大豆种子携带病原菌的状况,在生产上有较大的应用价值。[ Objectives ] In order to know the situation of soybean seed-borne pothogens in Huang-huai area, the soybean seed-borne pathogens were detected using loop-mediated isothermal amplification （LAMP）assays. [ Methods ] In this research, 13 species of seed-borne pothogens on soybean seed samples of 29 cultivars in Huang-huai area were detected using LAMP method, including Colletotrichum truncatum , C. gloeosporioide , Fusarium oxysporum , F. equiseti , F. proliferatum , F. culmorum , F. graminearurn , F. solani , Phomapsis longicolla , Rhizoctonia solani , Macrophomina phaseolina , Calonectria ilicicola , and Phytophthora sojae. [ Results ] There are 20 soybean cultivars earring 7 of these seed-borne pothogens, and the dominant population is C. truncatum, followed by P. longicolla, F. equiseti , F. culmorum , F. oxysporum , C. gloeosporioide , and F. proliferatum. The pathogens carried with seeds of different cuhivars are various, and the number of pathogens detected changes from 1 to 6. [ Conclusions ] A simple, rapid and sensitive method for detecting soybean seed-borne pothogens was developed in this study, and the situation of soybean seed-borne pothogens in Huang- huai area was revealed with the method.

关 键 词：大豆 种子带菌 环介导等温扩增技术 快速检测 

分 类 号：S565.1[农业科学—作物学]