芪苈强心胶囊对心肌梗死后心力衰竭大鼠肺结构重塑作用及机制  

Effects of Qili Qiangxin Capsule on Lung Structural Remodeling in Heart Failure Rats after Myocardial Infarction and Its Mechanism

作　　者：韩安邦 张健 赵明镜[4] 赵一舟[4] 何姚姚 张丹丹[1,2] 崔向宁[1] 

机构地区：[1]中国中医科学院广安门医院心内科,北京100053 [2]北京中医药大学研究生院,北京100029 [3]纳通医疗集团销售部,北京100082 [4]北京中医药大学附属东直门医院中医内科学教育部和北京市重点实验室,北京100700

出　　处：《中国中西医结合杂志》2016年第11期1329-1334,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81273945)

摘　　要：目的观察芪苈强心胶囊防治心肌梗死(心梗)后心力衰竭大鼠肺结构重塑的作用及机制。方法结扎大鼠冠脉左前降支,建立急性心梗模型,随机分为模型组(灌胃蒸馏水1 m L/100 g,n=13),芪苈强心组[中药组,灌胃芪苈强心胶囊药液1 g/(kg·d),n=9],另设假手术组(灌胃蒸馏水1 m L/100 g,n=10)。干预4周后,应用超声心动图检测大鼠的心功能;取肺组织采用HE染色、Masson染色方法观察肺的结构学变化;用Western blot方法、免疫组织化学法检测肺组织α-SMA、CollagenⅠ、TGF-β1、p-Smad3蛋白表达情况。结果与假手术比较,模型组EF、FS降低(P<0.05),LVIDd、LVIDs、EDV、ESV、α-SMA、CollagenⅠ、TGF-β1、pSmad3蛋白表达升高(P<0.05),模型组肌型血管比例及肺组织胶原面积升高(P<0.05)。与模型组比较,中药组EF、FS升高(P<0.05),LVIDs、ESV、肺α-SMA、CollagenⅠ、TGF-β1、p-Smad3蛋白表达降低(P<0.05),中药组肌型血管比例及肺组织胶原面积域降低(P<0.05)。结论芪苈强心胶囊可改善心梗后心力衰竭大鼠肺结构重塑程度,其作用机制可能通过调控TGF-β1/Smad3信号通路实现。Objective To observe the effect of Qili Qiangxin Capsule （QQC） in improving lung struc- tural remodeling on heart failure （HF） rats after myocardial infarction （MI） and to study its possible mecha- nism. Methods The proximal left anterior descending branch of coronary artery was ligated using a terylene suture to establish acute myocardial infarction （AMI） rat model. After successful AMI modeling rats were ran- domly divided into the model group （intragastrically administered with distilled water at 1 mL/100 g, n =13） and the QQC group （intragastrically administered with QQC at the daily dose of 1 g/kg, n =9）. And the sham-opera- tion group （intragastrically administered with distilled water at 1 mL/100 g, n =10） was also set up. After four weeks intervention heart functions of rats were detected using echocardiography. The pathological changes of lung structures were observed by HE and Masson staining method. Protein expressions of lung α-SMA, Colla- gen 1, TGF-β1, and p-Smad3 of the lung tissue were detected by immunohistochemistry and Western blot. Re- sults Compared with the sham-operation group, ejection fraction （EF） and fraction shortening （FS） de- creased （P 〈0.05）, protein expressions of lung left ventricular internal diastolic diameter （LVIDd）, left ventric- ular internal systolic diameter （LVIDs）, end diastolic volume （EDV）, end systolic volume （ESV）, s-smooth muscle actin （α-SMA）, Collagen I, tumor growth factor-β1 （TGF-β1）, and p-Smad3 increased （P 〈0.05） in themodel group. The muscularized small arteries ratio and collagen area of the lung tissue increased in the model group （P 〈0.05）. Compared with the model group, EF and FS increased （P 〈0.05）, protein expressions of LVIDs, ESV, （x-SMA, Collagen I, TGF-β1, and p-Smad3 decreased （P 〈0.05） in the QQC group. The muscular- ized small arteries ratio and collagen area of the lung tissue decreased in the QQC group （P 〈0. 05）. Conclusion QQC could impro

关 键 词：慢性心力衰竭 肺结构重塑 芪苈强心胶囊 TGF-β1/Smad3信号通路 

分 类 号：R259[医药卫生—中西医结合]