升清胶囊对胆固醇结石小鼠胆固醇及肝脏B类清道夫受体的影响  

Effect of Shengqing Capsule on Serum Cholesterol Content and Hepatic Scavenger Receptor Class B of Rats with Cholesterol Calculus

作　　者：李炯[1] 陆巍[2] 梁晓强[3] 林天碧[1] 蒋海涛[1] 

机构地区：[1]上海中医药大学附属龙华医院普外科,上海200032 [2]上海中医药大学附属龙华医院急诊科,上海200032 [3]上海中医药大学附属龙华医院中医外科研究所,上海200032

出　　处：《中国中西医结合杂志》2016年第11期1369-1372,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.81173267);上海市高级中西医结合人才培养项目(No.ZYSNXD012-RC-ZXY007);上海市自然科学基金资助项目(No.15ZR1441800);海派中医顾氏外科流派建设项目(No.ZYSNXD-CC-APGC-JD002);第五批全国老中医药专家学术经验继承工作项目

摘　　要：目的观察升清胶囊对胆固醇结石小鼠血清TC、LDL-C、HDL-C含量及肝脏B类清道夫受体—B类Ⅰ型清道夫受体(scavenger receptor BⅠ,SRBⅠ)和CD36的影响。方法将80只小鼠按随机数字表法分为正常组、模型组、西药组、中药组,每组20只。正常组喂饲普通饲料,其他3组喂饲致石饲料,各给药组同时灌胃相应药物[升清胶囊0.35 g/(kg·d)或熊去氧胆酸片39.55 mg/(kg·d)]7周,观察小鼠一般状态和成石率,检测小鼠血清TC、LDL-C、HDL-C含量(氧化酶法)及肝脏SRBⅠ和CD36蛋白表达(免疫印迹法)。结果正常组未形成结石,模型组15只形成结石,成石率为75%。与正常组比较,模型组小鼠血清TC与LDL-C含量及SRBⅠ、CD36蛋白表达升高,HDL-C含量降低(P<0.01)。与模型组比较,西药组和中药组成石率[西药组:7只(35%);中药组:6只(30%)]降低(P<0.05),TC与LDL-C含量及SRBⅠ、CD36蛋白表达降低(P<0.01),HDL-C含量升高(P<0.01)。与西药组比较,中药组TC含量及SRBⅠ、CD36蛋白表达降低(P<0.01)。结论升清胶囊可能通过降低肝脏SRBⅠ和CD36防治胆囊结石。Objective To observe the effect of Shengqing Capsule （SC） on serum contents of TC, LDL-C, and HDL-C, hepatic scavenger receptor B I （SRB ｜ ）, and CD36 in rats with cholesterol cal- culus. Methods Totally 80 mice were divided into 4 groups according to random number table, i.e., the normal group, the model group, the Western medicine （WM） group, and the Chinese medicine （CM） group, 20 in each group. Mice in the normal group were fed with common forage, while mice in the other 3 groups were fed with lithogenic diet. Mice in the CM group and the WM group were fed with SC （at the daily dose of 0.35 g/kg） and Ursodeoxycholic Acid Tablet （UDCA, at the daily dose of 39.55 mg/kg） re- spectively for 7 weeks. The general condition and gallstone formation rate were observed. Serum contents of TC, LDL-C, and HDL-C, and protein expressions of SBR I and CD36 were detected by oxidase meth- od and Western blot respectively. Results No gallbladder stone formed in the normal group, and gall- stone formed in 15 mice of the model group with gallstone formation rate of 75%. Compared with the nor- mal group, serum contents of TC and LDL-C and protein expressions of SRB I and CD36 increased, HDL-C content decreased in the model group （P 〈0.01 ）. The gallstone formation rate was 35% （7 mice） in the WM group and 30% （6 mice） in the CM group, lower than that of the model group （75% ; P 〈0.05）. Contents of TC and LDL-C, and protein expressions of SRB I and CD36 decreased, HDL-C content in- creased in the WM group and the CM group （P 〈0.01 ）. Compared with the WM group, TC content and protein expressions of SRB I and CD36 decreased in the CM group （P 〈0.01 ）. Conclusion SC could prevent and treat gallbladder stone possibly through lowering expression levels of SRB I and CD36.

关 键 词：升清胶囊 胆固醇结石 胆囊结石 B类清道夫受体 

分 类 号：R259[医药卫生—中西医结合]