四君子汤对溃疡性结肠炎模型小鼠claudin-1/3的影响  

Effect of Sijunzi Decoction on Expression of Claudin-1 and Claudin-3 in Mouse Model of Ulcerative Colitis

作　　者：何爱明[1] 张涛[2] HE Aiming ZHANG Tao(Department of Biochemistry, Fuqing Campus of Fujian Normal University, Fuqing 350300, China Department of Gastroencerology, Ruikang Hospital Affiliated with Guangxi University of Traditional Chinese Medicine ,Nanning 530011, China)

机构地区：[1]福建师范大学福清分校生化系,福清350300 [2]广西中医药大学附属瑞康医院消化科,南宁530011

出　　处：《医药导报》2016年第11期1190-1194,共5页Herald of Medicine

基　　金：广西卫生厅重点项目(2011041)

摘　　要：目的观察四君子汤对溃疡性结肠炎模型小鼠的治疗作用及可能机制,以及对结肠黏膜超微结构变化和结肠黏膜claudin-1、claudin-3蛋白及其mRNA表达的影响。方法将90只Balb/C小鼠分为正常对照组、模型对照组、美沙拉嗪组及四君子汤小、中、大剂量组。除正常对照组外,其余各组小鼠均自由饮用3%葡聚糖硫酸钠2周建立溃疡性结肠炎模型。自第3周起美沙拉嗪组予美沙拉嗪灌胃,四君子汤小、中、大剂量组分别灌胃给予四君子汤3.12,6.24,12.48 g·kg-1灌胃,每天1次,连续1周。透射电镜分析结肠黏膜超微结构变化,实时-聚合酶链反应(RT-PCR)及Western blotting技术检测结肠黏膜claudin-1、claudin-3蛋白及其mRNA表达。结果模型对照组小鼠结肠黏膜上皮细胞绒毛稀疏,上皮细胞连接增宽,杯状细胞减少,线粒体肿胀或空泡变,可见细胞凋亡小体;四君子汤各剂量组微绒毛形态较规则,排列整齐,杯状细胞较多,线粒体肿胀,嵴结构不清,个别内质网扩张。模型对照组小鼠结肠黏膜claudin-1、claudin-3蛋白及其mRNA表达减少;四君子汤各剂量组结肠黏膜claudin-1、claudin-3蛋白及其mRNA表达较模型对照组增加,差异有统计学意义(P<0.05),四君子汤各剂量组claudin-1、claudin-3蛋白及其mRNA表达与正常对照组比较,差异无统计学意义。结论四君子汤通过调控claudin-1、claudin-3蛋白变化,加速结肠黏膜上皮修复,促进溃疡愈合,发挥缓解溃疡性结肠炎的功效。Objective To explore the therapeutic effects of Sijunzi decoction in mice model of ulcerative colitis, including the ultrastructural changes in colon mucosa and the protein and mRNA expressions of claudin-1 and claudin-3. Methods Ninety Balb/c mice were divided into normal control group, model control group, mesalazine group and low, medium and high dose Sijurnzi decoction group.All mice were fed with 3% DSS solution for two weeks to establish the ulcerative colitis model except the normal control group. From the third week, the mesalazine group were treated with mesalazine, and Sijunzi decoction group were intragastric administrated once daily for 7 days. Ultra structural changes in colon mucosa were analyzed by transmission electron microscope and the expression of claudin-1 and claudin-3 was detected by Real-time PCR and Western-blot. Results The ulcerative colitis is characterized by sparsity of epithelial villus, widen of epithelial connection, reduction of goblet cells and swelling of mitochondria.The Sijunzi decoction groups were characterized by regular arrangement of microvillus and increment of goblet cells, as well as mitochondria swelling and rough endoplasmic reticulum dilatation. The expressions of claudin-1 and claudin-3 were decreased in model control group while they were increased in the Sijunzi Decoction group and the difference is significant （P〈0.05）. Expressions of claudin-1 and claudin-3 were not significantly different between normal control group and Sijunzi Decoction group. Conclusion Sijiunzi decoction has effects of ulcer colitis remission and intestinal epithelial restoration by regulating the expression of claudin-1 and claudin-3.

关 键 词：四君子汤 超微结构 结肠黏膜 CLAUDIN 结肠炎 溃疡性 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]