青花菜及近缘种属种质资源抗根肿病鉴定  

Identification of Germplasm Resistant to Clubroot(Plasmodiophora brassicae Woronin) in Broccoli(Brassica oleracea L. var.italica Plenck) and Its Relatives

作　　者：张小丽[1] 刘玉梅[1] 方智远[1] 杨丽梅[1] 庄木[1] 张扬勇[1] 李占省[1] 吕红豪[1] ZHANG Xiao-li LIU Yu-mei FANG Zhi-yuan YANG Li-mei ZHUANG Mu ZHANG Yang-yong LI Zhan-sheng LV Hong-hao(Institute of Vegetables and Flowers of Chinese Academy of Agricultural Sciences, Beijing 100081)

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081

出　　处：《植物遗传资源学报》2016年第6期1106-1115,共10页Journal of Plant Genetic Resources

基　　金：国家现代农业产业技术体系建设专项资金项目(CARS-25-A);国家科技支撑计划课题项目(2013BAD01B04);国家高新技术发展计划"863"项目(2012AA100105);农业部园艺作物生物学与种质创制重点实验室项目;中国农业科学院科技创新工程项目(CAASASTIP-IVFCAAS)

摘　　要：根肿病是十字花科蔬菜作物的主要病害之一,造成了蔬菜产业巨大经济损失。青花菜是一种重要的十字花科蔬菜,具有良好的防癌保健功效。近年来青花菜根肿病在我国浙江、云南等青花菜主产区发生日益严重,鉴定和筛选青花菜抗根肿病资源,从而培育抗病品种是防治该病最经济有效的方法。为挖掘和丰富可利用的根肿病抗源,本试验针对我国优势根肿菌小种—4号小种,利用苗期人工接种鉴定方法—伤根灌菌法对531份青花菜及其近缘种属材料进行了抗根肿病鉴定。结果显示,446份青花菜材料(其中高代自交系393份,杂交种53份)中缺乏高抗(HR)和抗病(R)材料,包括中抗(MR)材料5份,均为自交系,占供试种质的1.12%,感病(S)材料189份,占供试种质的42.38%,高感(HS)材料252份,占供试种质的56.50%。85份近缘种属材料(其中甘蓝9份,花椰菜32份,大白菜7份,芜菁4份,芥蓝12份,苤蓝8份,菜心8份,油菜2份,野生种2份,欧洲山芥1份)中,包括免疫(I)材料1份,高抗(HR)材料1份,抗病(R)材料5份,中抗(MR)材料2份,感病(S)材料39份,高感(HS)材料37份。总体上高抗青花菜材料缺乏,近缘种属材料中抗源材料比例略高,这些材料为青花菜及甘蓝类蔬菜抗根肿病病育种提供了抗性资源。Clubroot is a destructive disease of cruciferae vegetable crops,which causes great economic loss.Broccoli is an important cruciferae vegetable,which has good anti-cancer effects. In recent years,the incidence of clubroot is increasing in the main producing areas of broccoli,such as Zhejiang,Yunnan province. Cultivating resistant varieties based on identifying and screening clubroot-resistance accessions is the most cost-effective method of preventing the disease. To explore available resistant germplasm against No. 4 race,the mainstream Plasmodiophora brassicaerace race in China,531 broccoli accessions and their relatives were artificially inoculated using irrigating injured root method. The results suggested that 5 accessions（inbred lines） were middle resistant（MR）,accounting for1. 12%; 189 accessions were susceptible（S）,accounting for 42. 38%; 252 accessions were highly susceptible（HS）,accounting for 56. 50%; no accessions were highly resistant（HR） or resistant（R） among 446 broccoli accessions（including 393 inbred lines,53 hybrids）. Among 85 relatives（including 9 cabbage accessions,32 cauliflower,7 Chinese cabbage,4 turnip,12 chinese kale,8 kohlrabi,8 flowering chinese cabbage,2 oilseed rape,2 wild accessions,1 Barbarea vulgaris R. Br.）,1 accession showed immune（I）,1 highly resistance（HR）,5 resistant（R）,2middle resistance（MR）,39 susceptible（S） and 37 highly susceptible（HS）. In short,there were more resistant type lines（HR,R,and MR） in relatives than that in broccoli lines. These materials would provide clubroot-resistance source for breeding in Brassica oleracea L.

关 键 词：青花菜 近缘种属 根肿病 抗源筛选 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]