富γ-氨基丁酸水稻种质筛选及与子粒性状相关性分析  

Selection of Rice Germplasm with Rich γ-aminobutyric Acid and Correlation Analysis of the Content and Grain Traits

作　　者：王迎超[1] 王全兴[1] 王浩[1] 孙刘臣 张银霞[1] 田蕾[1] 杨淑琴[1] 李培富[1] WANG Ying-chao WANG Quan-xing WANG Hao SUN Liu-chen ZHANG Yin-xia TIAN Lei YANG Shu-qin LI Pei-fu(College of Agriculture, Ningxia University, Yinchuan 750021)

机构地区：[1]宁夏大学农学院,银川750021

出　　处：《植物遗传资源学报》2016年第6期1116-1122,共7页Journal of Plant Genetic Resources

基　　金：宁夏育种专项水稻新品种选育项目(2013NYYZ0302);国家自然科学基金项目(31360324)

摘　　要：利用高效液相色谱法,测定了108份水稻种质资源材料子粒中的γ-氨基丁酸含量,分析了不同种质资源材料间γ-氨基丁酸含量差异及与子粒性状的相关性,以γ-氨基丁酸含量差异较大的高粱稻-1与宁农黑粳配制杂交组合,测定亲本、杂交F1及216个F2子粒中的γ-氨基丁酸含量,分析其含量与相对胚重和子粒性状的相关性及变异系数,估算各性状的广义遗传力。结果表明：108份种质资源材料子粒中的γ-氨基丁酸含量变异范围为2.39-12.03 mg/100g,,平均含量为6.30±1.99 mg/100g,变异系数为31.59%;不同水稻种质资源材料子粒中的γ-氨基丁酸含量与粒厚、千粒重呈极显著的负相关;杂交F1子粒γ-氨基丁酸含量为8.39±0.11 mg/100g,介于双亲之间;F2单株子粒中的γ-氨基丁酸含量总体呈偏正态分布,且出现明显的超亲现象,说明水稻子粒中的γ-氨基丁酸含量是由多基因控制的数量性状遗传;杂交F2子粒中的γ-氨基丁酸含量与相对胚重呈极显著的正相关,与粒厚、千粒重呈极显著的负相关,与粒长呈显著的负相关;F2单株子粒中的γ-氨基丁酸含量、粒厚和千粒重的广义遗传力相对较高,分别为98.12%、91.99%、96.37%,在育种中对这些性状可进行早期选择。The content of γ-aminobutyric acid in grains of 108 rice germplasm resources was tested by high performance liquid chromatography method,the difference of the content of γ-aminobutyric acid in different germplasm resources and the correlation between the γ-aminobutyric acid content and grain traits were analyzed. The study made up a hybrid combination of Gaoliangdao-1/Ningnongheijing according to great different content of γ-aminobutyric acid,and tested the determination of the content of γ-aminobutyric acid in rice parents,hybrids F1 and 216 F2 grains,and analyzed the correlation between the content and relative embryo weight,grain traits and coefficient of variation,and estimated the broad heritability of the traits. The results showed that： the variation range of the γ-aminobutyric acid content of 108 germplasm resources was 2. 39-12. 03 mg/100 g,the average content was 6. 30 ±1. 99 mg/100 g,the variation coefficient was 31. 59%; the content of γ-aminobutyric acid in rice germplasm resources was extremely significant negative correlativity with grain thickness and 1000-grain weight; the content of γ-aminobutyric acid in the hybrids F1 grains was 8. 39 ± 0. 11 mg/100 g,which was between two parents; the content of γ-aminobutyric acid in hybrids F2 plant grains tended to skewed normal distribution and there was an obvious transgressive phenomenon,and showed that the content of γ-aminobutyric acid in rice grains was controlled by multiplegenes; the content of γ-aminobutyric acid in hybrids F2 grains was extremely significant positive correlativity with relative embryo weight,and was extremely significant negative correlativity with grain thickness and 1000-grain weight,and was significant negative correlativity with grain length; the γ-aminobutyric acid content,grain thickness and 1000-grain weight of F2 plant grains had relatively high broad heritability,respectively were 98. 12%,91. 99%,96. 37%,and these traits can be selected at early period in rice breeding.

关 键 词：水稻 Γ-氨基丁酸 子粒性状 广义遗传力 相关性 

分 类 号：S511[农业科学—作物学]