结球甘蓝根肿菌鉴定和种质抗性评价  

Identification of Plasmodiophora brassicae Wor. and Resistance Assessment of Cabbage(Brasisica oleracea var. capitata L.) Germplasm

作　　者：王神云[1] 吴强[2] 王红[1] 于利[1] 李建斌[1] 丁万霞[1] WANG Shen-yun WU Qiang WANG Hong YU Li LI Jian-bin DING Wan-xia(Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanfing 210014 Jiangsu Zhongjiang Seed Co. LTD, Nanjing 211500)

机构地区：[1]江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,南京210014 [2]江苏中江种业股份有限公司,南京211500

出　　处：《植物遗传资源学报》2016年第6期1123-1132,共10页Journal of Plant Genetic Resources

基　　金：农业部国家现代农业产业技术体系项目(CARS-25);江苏省科技支撑项目(BE2013429);江苏省农业科技自主创新基金项目[CX(13)2004]

摘　　要：采集湖北省长阳县火烧坪乡根肿病重发区的病土和病根,通过病原菌形态和PCR鉴定,确定是芸薹根肿菌,然后利用欧洲ECD鉴别系统确定生理小种为ECD17/31/13,此病原菌致病力极强。采用田间苗期人工接种鉴定,与田间成株期自然诱发鉴定相结合,对88份甘蓝种质进行抗性评价和筛选,结果表明:苗期获得1份高抗,7份抗病,17份耐病材料;成株期获得4份高抗,4份抗病,15份耐病材料。2个时期88份材料群体抗性鉴定级别基本一致,93.18%材料成株期病指比苗期高。CR21在2个时期均为高抗,抗性最强,表现稳定;CR55在苗期发病最严重,病指达到76.19,成株期为74.10;CR54在成株期发病最严重,病指达到81.54,苗期为75.97,2个时期发病率均达到100%。根肿病菌的鉴定和致病力的确定,及甘蓝种质抗性评价为抗病品种选育和抗病机理的研究奠定基础。Morphological identification and PCR confirmed that the Plasmodiophora brassicae is the pathogen caused cabbage clubroot. Using European ECD identification system,the pathogen was further identified as a physiological strain coded as ECD17/31/13. Field trial of 88 cabbage germplasm artificially inoculated by ECD17/31/13 was conducted in village Huoshaoping（HSh P）,Hubei Province,to assess their resistance nature to the clubroot. After evaluation,their resistant phenotypes were leveled as highly resistant（HR）,resistant（R）,tolerant（T） and susceptible（S）. The results showed that among the 88 cabbage germplasm engaged,one HR,seven R and seventeen T in seedling stage,and four HR,four R,fifteen T in plant stage. Germplasm CR21 was the HR material which performed the best in both stages stably. Materials CR55 and CR54 were found as the most susceptible. The disease index of CR55 and CR54 were 76. 19 and 75. 97 in seedling stage,and 74. 10 and 81. 54 in plant stage respectively,when the incidence rates of CR55 and CR54 were both 100%. The taxonomic and virulence evaluation on the pathogen of clubroot disease,and screening resistant germplasm in cabbage would provide clue to further understand the phytopathological mechanism underlying and material for plant breeding.

关 键 词：结球甘蓝 根肿菌 种质资源 抗性评价 

分 类 号：S436.35[农业科学—农业昆虫与害虫防治]