疏肝和胃方对内脏高敏感大鼠CaMKⅡ表达的影响  

Effects of Shugan Hewei Decoction on the expression of CaMKⅡ in visceral hypersensitive rats

作　　者：徐亭亭[1,2] 朱生樑[1] 张秀莲[1] 程艳梅[1] XU Ting-ting ZHU Sheng-liang ZHANG Xiu-lian CHENG Yan-mei(Department of Gastroenterology, Yueyang Hospital of Integrated Traditional and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 200437 Postgraduate School, Shanghai University of Traditional Chinese Medicine, Shanghai ,201203)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院消化科,上海200437 [2]上海中医药大学研究生院,上海201203

出　　处：《上海中医药杂志》2016年第11期74-77,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市科委科技支撑重大项目(13401902801)

摘　　要：目的观察疏肝和胃方对内脏高敏感钙调蛋白激酶Ⅱ(CaMKⅡ)表达的影响。方法健康雄性SD大鼠32只,随机分为模型组、疏肝和胃方组、雷贝拉唑组及空白对照组4组,每组8只。采用鸡卵清蛋白联合食管酸灌注建立内脏高敏感模型。免疫组化法观察各组大鼠脊髓CaMKⅡ含量的表达及阳性神经元个数。结果与空白对照组比较,模型组CaMKⅡ含量的表达、阳性神经元个数明显升高(P<0.05);与模型组比较,中药组、西药组CaMKⅡ含量的表达、阳性神经元个数明显降低,差异有统计学意义(P<0.01);在阳性神经元个数方面,中药组低于西药组(P<0.05);在CaMKⅡ表达方面,中药组与西药组比较,差异无统计学意义(P>0.05)。结论疏肝和胃方可能通过抑制Ca MKⅡ表达降低内脏高敏感。Objective To observe the effects of Shugan Hewei Decoction on the expression of Ca-（2＋）/calmodulin-dependent protein kinaseⅡ（CaMKⅡ） in visceral hypersensitive rats.Methods 32 healthy male SD rats were randomly divided into the model group,Shugan Hewei Decoction group,rabeprazole group and blank control group,eight rats in each group.The model of visceral hypersensitivity was established by the chicken ovalbumin combined with esophageal acid perfusion.The expression of CaMKⅡ in spinal cord and the number of positive neurons of rats in each group were observed by immunohistochemistry.Results Compared with the blank control group,the expression of CaMKⅡ and the number of positive neurons in the model group were increased significantly（P〈0.05）; compared with the model group,the expression of CaMKⅡ and the number of positive neurons in the Shugan Hewei Decoction group and rabeprazole group were decreased significantly,and the differences were statistically significant（P〈0.01）.The number of positive neurons in the Shugan Hewei Decoction group was less than that in the control group（P〈0.05）.There was no statistically significant difference on the expression of CaMK Ⅱ between the Shugan Hewei Decoction group and rabeprazole group（P〉0.05）.Conclusion Shugan Hewei Decoction could decrease the visceral hypersensitivity by the inhibition of CaMKⅡ expression.

关 键 词：疏肝和胃方 内脏高敏感 CaMKⅡ 

分 类 号：R285.5[医药卫生—中药学]