微小RNA-133/30C在周围神经纤维化过程中的变化及意义  

Expression and role of microRNA-13 and microRNA-30C in the peripheral nerve after chronic compression injury

作　　者：胡锐[1] 喻爱喜[1] 任义军[2] 严立[2] 易新成[2] 丁凡[2] Hu Rui Yu Aixi Ren Yijun Yan Li Yi Xincheng Ding Fan(Department of Orthopedics, Zhongnan Hospital, Wuhan University, Wuhan 430071, China Department of Orthopedics, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430033, Chin)

机构地区：[1]武汉大学中南医院骨科,430071 [2]华中科技大学同济医学院附属普爱医院骨科,武汉430033

出　　处：《中华实验外科杂志》2016年第11期2499-2503,共5页Chinese Journal of Experimental Surgery

基　　金：武汉市卫计委临床医学科研项目（WX13817）;湖北省卫计委一般项目课题（WJ2015MB155）

摘　　要：目的 探讨大鼠坐骨神经慢性卡压损伤后其卡压神经段内微小RNA（miRNA,miR）-133及miR-30C表达变化及其意义.方法 将50只成年雄性SD大鼠随机分成A、B两组：A组（假手术组）：仅分离暴露坐骨神经;B组（卡压组）：采用Mackinnon建立的坐骨神经卡压模型方法对大鼠右侧后腿坐骨神经行硅胶管卡压术.于卡压术后2、4、6、8、10周时间点随机取A、B组大鼠各5只,取其卡压段坐骨神经行组织形态学,反转录-聚合酶链反应（RT-PCR）定量测定miR-133及miR-30C、结缔组织生长因子（CTGF）及Ⅰ型胶原蛋白（COL-Ⅰ）含量.结果 坐骨神经慢性卡压损伤后,卡压段神经组织胶原纤维增生,CTGF及COL-Ⅰ表达升高的同时,伴有miR-133及miR-30C表达下降;A组和B组神经中miR-133表达分别为：2周组：7.025 8±0.0062、4.645 7±0.145 3;4周组：6.2849±0.0048、3.1259±0.014 8;6周组：7.7253±0.008 1、4.5846±0.2148;8周组：6.562 6±0.0048、5.254 3±0.158 9;10周组：7.384 1±0.0018、5.014 5±0.348 6,两组比较差异有统计学意义（P＜0.05）.结论 慢性卡压损伤可致周围神经发生纤维化病变,CTGF表达升高,miR-133及miR-30C在此过程中表达下调,提示miR-133及miR-30C可一定程度调控CTGF,其在周围神经纤维化过程起重要作用.Objective To investigate the effects of microRNA （miR）-13 and miR-30C on the chronic peripheral nerve compression injury and explore the function of miR-13 and miR-30C in peripheral nerve compression injury and repair.Methods 50 adult male SD rats were randomly divided into group A and B：group A （sham-operated group）：only exposed the sciatic nerve;group B （compression group）：undergone sciatic nerve entrapment operation on the right hind leg according to the method which Mackinnon adopted when he established the model of chronic sciatic nerve compression.Histomorphology,immunohistochemistry and reverse transcription-polymerase chain reaction （RT-PCR） were performed to observe the morphological changes of the compressed nerve tissue and to determine the level of miR-13 and miR-30C,connective tissue growth factor （CTGF）,collagen-Ⅰ （COL-Ⅰ）,2,4,6,8,10 weeks after the surgery respectively.Results After sciatic nerve compression,the collagen in nerve increased;The expression of CTGF and COL-Ⅰ in sciatic nerve of compressed group increased;In the meanwhile,the expression of miR-13 and miR-30C in sciatic nerve of compressed group reduced,which is statistically different compared with the sham-operation group （P ＜ 0.05）.Conclusion Peripheral nerve fibrosis can be caused by chronic nerve compression.The expression of CTGF and COL-Ⅰ in sciatic nerve increased and miR-13 and miR-30C reduced in the pathophysiological process,which suggests that miR-13 and miR-30C plays an important role in the process of neural injury and fibrosis.

关 键 词：周围神经 微小RNA 神经卡压综合征 慢性卡压 

分 类 号：R692.330.2[医药卫生—泌尿科学]