蒙药大栀子超微饮片的质量标准研究  

Study on the Quality Standard of Ultramicro Decoction Piece of Meng Medicine Gardenia jasminoides

作　　者：白万富[1] 鞠爱华[2] 张静 蔡丽娟[2] 庄志鹤 

机构地区：[1]内蒙古科技大学包头医学院,内蒙古包头014040 [2]内蒙古医科大学药学院,呼和浩特010059

出　　处：《中国药房》2016年第33期4689-4691,共3页China Pharmacy

基　　金：国家科技支撑计划课题(No.2012BAI28B01)

摘　　要：目的：建立蒙药大栀子超微饮片的质量标准。方法：观察超微饮片的显微鉴别特征;采用薄层色谱法（TLC）对超微饮片进行定性鉴别;检测超微饮片水分、灰分、浸出物量;采用高效液相色谱法测定超微饮片中栀子苷的含量：色谱柱为Kromasil C18,流动相为乙腈-水（15∶85,V/V）,流速为1.0 ml/min,检测波长为238 nm,柱温为30℃,进样量为10μl。结果：超微饮片细胞已完全破壁,粒径为5-60μm。超微饮片TLC斑点清晰,分离良好。超微饮片水分为3.89%-5.55%,灰分为3.13%-5.22%,酸不溶性灰分为0.16%-0.80%,浸出物为29.2%-43.4%。栀子苷检测质量浓度线性范围为1.56-7.82μg/ml（r=0.999 9）;精密度、稳定性、重复性试验的RSD〈2.0%;加样回收率为100.17%-102.04%（RSD=1.07%,n=6）。结论：该研究所建标准可用于蒙药大栀子超微饮片的质量控制。OBJECTIVE： To establish the quality standard of ultramicro decoction piece of Meng medicine Gardenia jasminoides. METHODS： Microscopic identification character of the ultramicro decoction piece was observed; TLC was adopted for its qualitative identification; moisture, ash, extract were detected; HPLC was used for the content determination of geniposide： the column was Kromasil C18 with mobile phase of acetonitrile-water （ 15 ： 85, V/V） at a flow rate of 1.0 ml/min, detection wavelength was 238 nm, column temperature was 30 ℃, and injection volume was 10 μl. RESULTS ： Cell wall was completely broken and the particle sizes were 5-60 μm. TLC spots were clear and well separated. The moisture was 3.89%-5.55%, ash was 3.13%-5.22%, acid-insoluble ash was 0.16%-0.80%, and extract was 29.2%-43.4%. The linear range of geniposide was 1.56-7.82 μg/ml （r=0.999 9） ; RSDs of precision, stability and reproducibility tests were lower than 2.0% ; recovery was 100.17%-102.04% （RSD= 1.07%, n=6）. CONCLUSIONS： The standard can be used for the quality control of ultramicro decoction piece of Meng medicine G. jasminoides.

关 键 词：蒙药大栀子 超微饮片 栀子苷 质量标准 

分 类 号：R282.5[医药卫生—中药学]