磁性抗体转染结肠癌干细胞的最佳浓度探索  

Optimal concentration of magnetic antibodies transfected into colon cancer stem cells in vitro

作　　者：王正雄[1] 刘敏[1] 陈华英[2] 梅雪芊 李学红[1] 李强[1] 谭书德[1] 刘元兵[1] 赵潇[1] Wang Zhengxiong Liu Min Chen Huaying Mei Xueqian Li Xuehong Li Qiang Tan Shude Liu Yuanbing Zhao Xiao(Department of Radiology Department of Obstetrics and Gynecology Department of Information, the People＇s Hospital of Qijiang District)

机构地区：[1]重庆市綦江区人民医院放射科,重庆401420 [2]重庆市綦江区人民医院妇产科,重庆401420 [3]重庆市綦江区人民医院信息科,重庆401420

出　　处：《重庆医科大学学报》2016年第10期1051-1055,共5页Journal of Chongqing Medical University

基　　金：重庆市綦江区科委资助项目(编号:QJ[2015]124)

摘　　要：目的：通过对不同质量浓度的磁性抗体体外转染结肠癌干细胞后的系列研究,探索最佳转染浓度。方法：将磁性抗体含铁浓度分别设定为5、10、25、50、75、100 mg/L,分别转染结肠癌干细胞,采用普鲁士蓝染色观察细胞内铁颗粒,原子吸收光谱仪法测定细胞铁含量并检测细胞活性,MR成像检测细胞信号强度变化。结果：各组细胞内铁含量组间比较采用单因素方差分析,组间差异具有统计学意义（F=71.709,P=0.000）,在α=0.05的检验水准下,可以认为各组间均数不完全相同,进一步采用单因素方差分析LSD法（双侧检验）进行组间均数多重比较,结果显示,50 mg/L组与5 mg/L组、10 mg/L组、25 mg/L组相比,差异均有统计学意义（P=0.000,P=0.000,P=0.000）,但与75 mg/L组、100 mg/L组比较,差异均无统计学意义（P=0.607,P=0.419）。细胞活性检测组间比较采用单因素方差分析,组间差异具有统计学意义（F=69.306,P=0.000）,在α=0.05的检验水准下,可以认为各组间均数不完全相同,进一步采用单因素方差分析LSD法（双侧检验）进行组间均数多重比较,结果显示,50 mg/L组与75mg/L比较,细胞活性差异无统计学意义（P=0.134）,与5 mg/L组、10 mg/L组、25 mg/L组、100 mg/L组比较,差异均有统计学意义（P=0.000,P=0.000,P=0.000,P=0.008）。T2WI信号强度采用单因素方差分析进行组间比较,组间差异具有统计学意义（F=3 180.748,P=0.000）,在α=0.05的检验水准下,可以认为各组间均数不完全相同,进一步采用单因素方差分析LSD法（双侧检验）进行组间均数多重比较,结果显示,50 mg/L组与75 mg/L、100 mg/L各组信号强度之间差异无统计学意义（P=0.945,P=0.960）,与空白对照组、细胞对照组、5 mg/L、10 mg/L、25 mg/L各组信号强度相比,差异均有统计学意义（P=0.000,P=0.000,P=0.000,P=0.000,P=0.000）。由此可见,磁性抗体含铁浓度在一定范围内（5~25 mg/L）增加,结肠癌干Objective：To explore the optimal transfection concentration through the study of the different mass concentrations of magnetic antibody in vitro transfection of colon cancer stem cells. Methods：The magnetic antibodies at an iron concentration of 5,10,25,50,75,100 mg/L were transfected into colon cancer stem cells. The distribution and content of iron particles in colon cancer stem cells were determined by Prussian blue staining,atomic adsorption spectrometer. Cell viability was observed and the changes of signal intensity were measured by magnetic resonance imaging（MRI）. Results：Results of single factor analysis of variance showed that there were statistically significant differences between each group in the iron content of cells（F=71.709,P=0.000）. It can be considered that the mean of each group was not exactly the same at the test level of α=0.05. Multiple comparisons were made between groups by the single factor analysis of variance LSD method（two-sided）,the result shows that the difference between 50 mg/L group and 5mg/L group,10 mg/L group,25 mg/L group was statistically significant（P=0.000,P=0.000,P=0.000）,but the difference was not statistically significant between 75 mg/L group and 100mg/L group（P=0.607,P=0.419）. Cell activity detection was compared using single factor analysis of variance,and the difference between each group was statistically significant（F =69.306,P =0.000）. It can be considered that the mean of each group was not exactly the same at the test level of α=0.05. Multiple comparisons were made between groups by the single factor analysis of variance LSD method（two-sided）;the result showed that the difference between50 mg/L group and 75 mg/L group was not statistically significant（P=0.134）;but the difference was statistically significant between 5mg/L group,10 mg/L group,25 mg/L group and 100 mg/L group（P=0.000,P=0.000,P=0.000,P=0.008）. T2 WI signal intensity was compared using single factor analysis of variance,and the difference between each gro

关 键 词：磁性抗体 肿瘤干细胞 结肠癌 最佳浓度 

分 类 号：R378.1[医药卫生—病原生物学]