过氧化氢清除剂对H_2O_2处理下中国石竹幼苗不同器官抗氧化系统的影响  

Influence of Hydrogen Peroxide Traps on Antioxidant System in Different Organs of Dianthus chinensis L.Under H_2O_2 Treatment

作　　者：张倩倩[1] 蔚潇 贺学勤[1] ZHANG Qianqian YU Xiao HE Xueqin(Agronomy Faculty,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010019)

机构地区：[1]内蒙古农业大学农学院,内蒙古呼和浩特010019

出　　处：《北方园艺》2016年第22期82-86,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(31260486);中国农业大学-内蒙古农业大学合作基金资助项目(ZN201102)

摘　　要：以中国石竹为试材,采用25mmol·L^(-1)二甲基硫脲(dimethylthiourea,DMTU)和3mmol·L^(-1)二苯基氯化碘盐(diphenyleneiodonium chloride,DPI)对其幼苗预处理2h后再置于5mmol·L^(-1) H_2O_2中处理6h,测定DMTU和DPI预处理对H_2O_2处理下中国石竹幼苗不同器官的H_2O_2和O·2含量以及相关清除酶活性的方法,探究减少H_2O_2含量对氧化胁迫下石竹幼苗生理活性的影响,以期为石竹抗逆栽培提供理论依据。结果表明:25mmol·L^(-1) DMTU和3mmol·L^(-1) DPI预处理降低了正常条件下叶片和根的H_2O_2和O·2含量,且在根中达显著差异水平(P<0.5);5mmol·L^(-1)的外源H_2O_2处理显著增加了叶中H_2O_2含量和根中O·2含量(P<0.5),25mmol·L^(-1) DMTU和3mmol·L^(-1) DPI预处理则降低了5mmol·L^(-1) H_2O_2处理下叶片和根部的H_2O_2和O·2含量;5mmol·L^(-1)的外源H_2O_2处理显著增加了叶片SOD活性和根部SOD、APX活性(P<0.5),25mmol·L^(-1) DMTU和3mmol·L^(-1) DPI预处理降低了5mmol·L^(-1)H_2O_2处理下石竹叶中SOD、APX活性,且25mmol·L^(-1) DMTU预处理显著降低了根部的APX活性(P<0.5),表明降低H_2O_2和O·2含量可降低相应清除酶的活性。因此,外施DMTU和DPI能不同程度缓解H_2O_2处理导致的内源H_2O_2和O·2含量的积累,且25mmol·L^(-1) DMTU清除H_2O_2的效果比3mmol·L^(-1) DPI好。Taking Dianthus chinensis L. as material,with 25 mmol·L-1 DMTU and 3 mmol·L-1 DPI for 2 hours were pretreated and 5 mmol·L-1 H2O2 for 6 hours were treated, then the contents of H2O2 and O2- and related enzyme activity were measured,the influence of reducing H2O2 content on physiological activity under oxidative stress in Dianthus chinensis L. seedlings were studied, in order to provide theoretical basis for cultivation of Dianthus chinensis L. under adversity. The results showed that the contents of H2O2 and O2- in the leaves and roots were reduced by pretreatment of 25 mmol·L-1 DMTU and 3 mmol·L-1 DPI under normal conditions and levels of reduce were significant in the roots （P〈0. 5）;treatment with 5 mmol·L-1 H2O2 led to the significant increase of the content of H2O2 in leaves and the content of O2- in roots （P〈0. 5） ,but pretreatment with 25 mmol·L-1 DMTU and 3 mmol·L-1 DPI declined both in the two organs;the enzyme activities of SOD in leaves and SOD,APX in roots increased significantly under 5 mmol·L-1 H2O2 treatment （P 〈 0. 5 ）, but the enzyme activities of SOD and APX in leaves were declined by pretreatments of 25 mmol·L-1 DMTU and 3 mmol·L-1 DPI and the enzyme activity of APX in roots was significantly declined by pretreatments of 25 mmol·L-1 DMTU （P〈0. 5） ,it suggested that the contents of H2O2 and O2- had positive relations with the activities of their scavenger enzymes. Therefore, DMTU and DPI could alleviate the accumulation of H2O2 and O2-content induced by H2O2 treatment with different levels,and the former had the better effect of alleviation.

关 键 词：中国石竹 过氧化氢 DMTU DPI 清除酶 

分 类 号：S681.5[农业科学—观赏园艺]