CD11c在新生儿及成人指皮组织表达的免疫组织化学研究  

Immunohistochemistry Study of Expression of CD11c In Finger Tissues of Neonates and Adults

作　　者：张洁[1] 徐长福[2] 莫立平[2] 孙颖[2] 韩水平[2] 赵文宝[2] 

机构地区：[1]西藏民族大学医学部基础医学院,陕西咸阳712082 [2]西安交通大学医学部基础医学院病理学系,陕西西安710061

出　　处：《现代生物医学进展》2016年第31期6038-6041,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(30370544);陕西省自然科学基金项目(2002C233)

摘　　要：目的:研究新生儿及成人指皮组织CD11c阳性细胞的数量、形态和分布情况。方法:取7例尸检新生儿及5例成人无名指指皮组织样本,CD11c标记,行免疫组织化学染色,光镜观察阳性细胞的表达、分布情况并进行统计学分析。结果:人指皮组织中,CD11c阳性细胞和阳性突起呈棕褐色,胞体大小不等、形态多样;胞核呈圆形或椭圆形,突起长短粗细不等,可相互连接。阳性细胞呈散在或灶带状分布,灶带状分布常见于表皮乳头周围、血管神经分叉周围,血管外膜、环层小体被囊结缔组织内可见散在的CD11c阳性细胞。新生儿组CD11c阳性细胞的表达率为(31.0±9.4)%,高于成人组(6.0±2.7)%(P<0.01)。结论:CD11c分子可能也是人指皮组织细胞分化过程中的阶段性标志物,其表达量的降低与年龄的增长有关。Objective： To study the form, number and distribution of CD11c positive cells in the finger tissues of neonates and adults. Methods： Autopsy samples of ring finger tissues of seven neonates and five adults were stained with the method of immunohistochemistry stained with CD11c, which were then observed by light microscope and with statistical analysis. Results： CD11c＋ cells and processes were colored with brown, which were different in sizes and shapes with their nucleus round or ellipse and with their processes different in length and thickness, which also can be connected with each other. CD11c＋ cells at scattering or focal or band- like distribution could be seen in finger tissues, including the surrounding of epidermal pinning processes and in the branching of vessels and nerves.CD11c＋ cells at scattering distribution could also be seen in adventitia and capsule tissues of Pacinian corpuscles. The expression ratio of CD11c＋ cells in neonates group is（31.0±9.4） %, which is, higher than that of adults group（6.0±2.7） %（P〈0.01）. Conclusion： CD11c molecule is likely to be the periodic markers in the cell differentiation process of the finger skin tissue in human, the decreasing of CD11c expression in human finger skin is related to the age growth.

关 键 词：新生儿 成人 无名指指尖组织 CD11C 树突状细胞 分化 免疫组织化学 

分 类 号：R-33[医药卫生] R722