纳米铂黑作为电极镀层的细胞毒性评价  

Evaluation of Cytotoxicity of Nanometer Platinum Black as Electrode's Cladding Material

作　　者：许栋岳[1] 刘景全[2] 孙雅静[3] 芮岳峰[2] 李克勇[3] 

机构地区：[1]赤峰学院附属医院,内蒙古赤峰024050 [2]上海交通大学微纳科学技术研究院,上海200240 [3]上海交通大学附属第一人民医院,上海200080

出　　处：《现代生物医学进展》2016年第32期6226-6230,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(60876082;81070779)

摘　　要：目的：探讨将纳米铂黑作为电极镀层的细胞毒性,初步评估铂黑电镀电极长期植入生物体内的安全性。方法：分别改变混悬液浓度（0.1 mg、0.2 mg、0.3 mg/m L铂黑混悬液）对L-929成纤维细胞培养24 h、48 h、72 h,显微镜下观察细胞形态变化并通过MTS法计算细胞相对增殖率,对细胞毒性进行评级。结果：0.2 mg/m L、0.3 mg/m L的铂黑混悬液中L-929成纤维细胞变成圆形、胞核固缩,细胞稀少,贴壁差;在0.1 mg/m L的铂黑混悬液中上述细胞形态变化则较轻微。0.1 mg/m L的铂黑混悬液细胞毒性为0~2级,且随时间延长而毒性逐渐减弱并呈现出无细胞毒性;0.2 mg/m L、0.3 mg/m L的铂黑混悬液细胞毒性为3~4级,不随时间变化。结论：纳米铂黑其质量体积浓度低于0.1 mg/m L时具有较好的生物相容性。Objective： To evaluate cytotoxicity of nanometer platinum black as an electrode cladding material and to assess biological security of platinum black electroplating electrode in long-term implantation. Methods： L-929 fibroblasts were cultured with suspension liquid of the nanometer platinum black in different concentrations（0.1 mg, 0.2 mg and 0.3 mg/m L）. After 24 h, 48 h and72 h,cells’ morphous were observed by microscope, and relative growth rates of cells were calculated via MTS for grading cytotoxicity.Results： L-929 fibroblasts grown into circle, pyknotic nucleus, decreased markedly and mal-adherence in the platinum black suspension liquid groups（0.2 mg/m L and 0.3 mg/m L）, but all above morphology changes of cells were slight in the 0.1 mg/m L platinum black suspension liquid. The cytotoxicity of platinum black suspension liquid group（0.1 mg/m L） was 0 to 2 degree, with time extended cytotoxicity weakened gradually and showed no cytotoxicity. However, the cytotoxicity of groups（0.2 mg/m L, 0.3 mg/m L） were 3 to 4degree and did not change. Conclusion： Nanometer platinum black has good biocompatibility in the mass volume concentration less than0.1 mg/m L.

关 键 词：铂黑 细胞毒性 MTS法 

分 类 号：R135[医药卫生—劳动卫生] R318.08[医药卫生—公共卫生与预防医学]