presenilin-1/presenilin-2双基因敲除小鼠的寿命观察  

Investigation on life span of presenilin-1/presenilin-2 conditional double knockout mice

作　　者：唐红 唐晓琴 邓飞[2] 李昕 陈波 唐明希 阮思蓓 

机构地区：[1]西南医科大学附属医院病理科,四川泸州646000 [2]南方医科大学附属深圳医院病理科,广东深圳518102 [3]西南医科大学神经解剖教研室,四川泸州646000

出　　处：《中国比较医学杂志》2016年第11期6-10,共5页Chinese Journal of Comparative Medicine

基　　金：四川省科技厅科技支撑计划(2012SZ0172);四川省教育厅重点项目(16ZA0196);西南医科大学-泸州市科技局联合资助项目(2015LZCYD-S06(6/11));西南医科大学重点项目(2015-YJ002);西南医科大学附属医院科研项目(15056;15087)

摘　　要：目的观察Presenilin-1/Presenilin-2双基因敲除小鼠(dKO mice)的生存曲线,为进一步繁殖及使用该模型对阿尔茨海默病(AD)进行研究提供实验设计等提供参考。方法将dKO mice雌性50只,雄性30只,及野生型同系小鼠雌、雄各20只分为4组,观察正常喂养情况下4组间小鼠2年以内各自的生存情况及寿命,绘制生存曲线进行生存分析。结果 dKO mice随着时间的延长,雌、雄性生存概率分别低于野生型雌、雄性小鼠,差异均有统计学意义(P<0.001,P<0.001;P<0.001;P<0.001);dKO mice雌、雄性之间的生存概率差异无统计学意义。结论条件性敲除presenilin-1与presenilin-2双基因可能会缩短小鼠的寿命,其生存概率显著低于野生型小鼠,而dKO mice雌、雄性之间的生存概率则无统计学差异。本实验结果可为借助此模型的实验设计应用提供有益参考。Objective To analyze the survival curves of presenilin-1 /presenilin-2 conditional double knockout mice（ dKO mice）,and provide a reference for further breeding of this strain of mice and studying the pathogenesis of Alzheimer＇s disease（ AD）. Methods dKO mice（ 50 females and 30 males） and wild-type controls（ 20 females and 20males） were included in this study. The survival of all the mice was observed under the same condition of feeding and nutrition for two years. Life span of each mouse was calculated,survival curves were drawn,and their survival was analyzed. Results With the passing of time, the survival probability was decreased in dKO mice. The survival probabilities of female and male dKO mice were significantly worse than that of wild-type controls,respectively（ P 0. 001,P 0. 001; P 0. 001,P 0. 001）. No significant difference was observed between the males and females of dKO mice. Conclusions Double knockout presenilin-1 and presenilin-2 genes may shorten the life span of mice,but there is no significant gender difference between the males and females of dKO mice.

关 键 词：dKO MICE 条件性基因敲除 小鼠 寿命 

分 类 号：R332[医药卫生—人体生理学]