不同剂量活血、破血中药对动脉粥样硬化小鼠主动脉PTGS2、PADI4、ITGAM基因表达的影响  

Effects of different doses of blood circulation and traditional Chinese medicine on the expression of PTGS2,PADI4 and ITGAM in aorta of atherosclerotic mice

作　　者：李杨[1] 周岚[1] 汪典[1] 卢青[2] 王敏[2] 陈旭[2] 罗尧岳[1] 谢海波[2] 

机构地区：[1]湖南中医药大学,410208 [2]湖南中医药大学第一附属医院,410007

出　　处：《疑难病杂志》2016年第11期1182-1186,共5页Chinese Journal of Difficult and Complicated Cases

基　　金：国家自然科学基金资助课题(81273752);2013年度高等学校博士学科点专项科研基金资助课题(20134323120007)

摘　　要：目的观察不同剂量活血中药(当归、川芎)、破血中药(三棱、莪术)对动脉粥样硬化(AS)小鼠主动脉环氧合酶-2(PTGS2)、肽酰基精氨酸脱亚胺酶(PADI4)、ITGAM基因表达的影响。方法选取64只5周龄ApoE基因敲除小鼠随机分为6组,即模型对照组(模型组)、阿托伐他汀钙组(他汀组)、低剂量活血药组(低活血组)、低剂量破血药组(低破血组)、高剂量活血药组(高活血组)、高剂量破血药组(高破血组),制备AS模型。模型制备成功后,连续灌胃给药8周,模型组灌服等体积蒸馏水。选用RT-PCR检测主动脉组织PTGS2、PADI4及TTGAM基因mRNA表达并分析其结果。结果与模型组比较,各药物组均能显著下调PTGS2、PADI4、ITGAM基因表达水平(P<0.01)。(1)在下调PTGS2基因表达水平方面,破血组优于活血组(P<0.01),高剂量组优于低剂量组(P<0.01),他汀组优于活血组、破血组(P<0.01);(2)在下调PADI4基因表达水平方面,破血组优于活血组(P<0.01),高剂量组优于低剂量组(P<0.01),他汀组优于活血组、破血组(P<0.01);(3)在下调ITGAM基因表达水平方面,高活血组优于高破血组(P<0.01),低活血组与低破血组作用相当(P>0.05),高剂量组优于低剂量组(P<0.01),他汀组优于活血组、破血组(P<0.01)。结论活血中药、破血中药可通过下调主动脉PTGS2、PADI4及ITGAM基因表达水平,从而起到抗动脉粥样硬化的作用。Objective To observe the effect of different doses of Chinese herbal medicine（Chinese angelica,Chinese medicine（Chuan Xiong）,broken blood Sanleng and Ezhu） on atherosclerosis（AS） in mouse aorta cyclooxygenase 2（PTGS2）,peptidyiarginine deiminase（PADI4） and ITGAM gene expression.Methods Sixty four ApoE gene deficient mice atherosclerosis（AS） models of five weeks were randomly divided into 6 groups,the model group,atorvastatin group（the statin group）,low dose of blood activating group（low blood activating group）,low dose of blood breaking group（low blood breaking group）,high dose of blood activating group（high blood activating group）,high dose of blood breaking group（high blood breaking group）.After successful modeling,drug gastric perfusion for 8 weeks,the model group was filled with distilled water,then,the mice were killed.PTGS2,PADI4 and ITGAM gene mRNA expression of the aortic tissue were tested with RT PCR method.Results Compared with model group,the PTGS2,PADI4 and ITGAM gene expression levels（P〈0.01）were significantly reduced in each drug group.（1） in the down-regulation of PTGS2 gene expression level,blood group than blood group（P〈0.01）,the high dose group than in low dose group（P〈0.01）,atorvastatin group was better than that of blood group and blood breaking group（P〈0.01）;（2） in the down-regulation of PADI4 gene expression level,blood breaking group is better than the live blood group（P〈0.01）,the high dose group than in low dose group（P〈0.01）,atorvastatin group was better than that of blood group and blood breaking group（P〈0.01）;（3） in the down-regulation of ITGAM gene expression level,high blood group is better than the high broken blood group（P〈0.01）.Low blood group and low blood group when（P〉0.05）,the high dose group than in low dose group（P〈0.01）,atorvastatin group was better than that of blood group and blood breaking group（P〈0.01）.Conclusion Promoting blood circulation to traditio

关 键 词：动脉粥样硬化 活血中药 破血中药 环氧合酶-2 肽酰基精氨酸脱亚胺酶-4 ITGAM 小鼠 

分 类 号：R285.5[医药卫生—中药学]