纳米二氧化钛皮肤暴露对小鼠脑和血管的毒性研究  

The Toxicity of Nano-TiO_2 on Brain and Blood Vessel of Mice by Dermal Exposure

作　　者：郭晴[1] 刘志敏[1] 李潇潇[1] 赵云[1] 沈世平[1] 蔡洁[1] 袁均林[1] 杨旭[1] 李睿[1] 

机构地区：[1]华中师范大学生命科学学院湖北省遗传调控和整合生物学重点实验室,湖北武汉430079

出　　处：《化学与生物工程》2016年第11期15-20,26,共7页Chemistry & Bioengineering

基　　金：国家自然科学基金重点项目(21577045)

摘　　要：为研究纳米二氧化钛(nano-TiO_2)对小鼠脑和血管的毒性,将56只小鼠随机分为8组,每组7只,分别为control、solvent control、4mg·kg^(-1) nano-TiO_2、20mg·kg^(-1) nano-TiO_2、100mg·kg^(-1) nano-TiO_2、500mg·kg^(-1) nanoTiO_2、100mg·kg^(-1)维生素E(VE)+100mg·kg^(-1) nano-TiO_2和100mg·kg^(-1) VE+500mg·kg^(-1)nano-TiO_2,采用皮肤涂抹的方式,对小鼠进行连续42d暴露。结果表明:4mg·kg^(-1) nano-TiO_2和20mg·kg^(-1) nano-TiO_2对小鼠的大脑毒性和血管损伤较低,100mg·kg^(-1) nano-TiO_2和500mg·kg^(-1) nano-TiO_2对小鼠的大脑毒性和血管损伤明显,暴露中VE的添加对nano-TiO_2给脑和血管造成的损伤具有一定的缓解作用。初步证明nano-TiO_2皮肤暴露在一定剂量下可对小鼠脑和血管引起毒性作用,其原因可能为氧化损伤。To investigate toxic effects of nano-TiO2 on brain and blood vessel of mice,56 BALB/c mice were randomly divided into 8 groups,including control, solvent control,4 mg·kg^-1 nano-TiO2,20 mg·kg^-1 nano- TiO2,100 mg ·kg^-1 nano-TiO2,500 mg·kg^-1 nano-TiO2,100 mg ·kg^-1 VE＋100 mg ·kg^-1 nano-TiO2 and 100 mg ·kg^-1 VE＋500 mg ·kg^-1 nano-TiO2 ,with 7 mice in each group. Mice were continuously exposed to nano-TiO2 by dermal exposure for 42 days. The results showed that, the brain and blood vessel of mice in 4 mg ·kg^-1 nano-TiO2 and 20 mg ·kg^-1 nano-TiO2 groups were slightly damaged,while obviously damaged in 100 mg·kg^-1 nano-TiO2 and 500 mg·kg^-1 nano-TiO2 groups,and vitamin E could relieve that injury to a certain extent. This study showed nano-TiO2 had toxic effects on brain and blood of mice by dermal exposure at a dose of nano-TiO2 and oxidative damage might be one of the reasons.

关 键 词：纳米二氧化钛 毒性 小鼠 皮肤暴露 

分 类 号：R114[医药卫生—卫生毒理学]