核壳型单磷酸腺苷分子印迹聚合物的制备  

Preparation of Core-Shell Adenosine-5′-Monophosphate Molecularly Imprinted Polymer

作　　者：龚霞[1] 胡莹莹[1] 尤祥宇[1] 谷晶[2] 邓教宇[2] 谢卫红[1] 

机构地区：[1]湖北工业大学生物工程与食品学院,湖北武汉430068 [2]中国科学院武汉病毒研究所中国科学院农业与环境微生物学重点实验室,湖北武汉430071

出　　处：《化学与生物工程》2016年第11期27-31,共5页Chemistry & Bioengineering

基　　金：中国科学院病毒研究所国家重点实验室开放研究基金(20875024)

摘　　要：以聚苯乙烯微球(CP)为内核、单磷酸腺苷(AMP)为模板、丙烯酰胺(AM)为功能单体、乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,制备了核壳型单磷酸腺苷分子印迹聚合物(AMP-MIP),讨论了不同洗脱方式、洗脱溶剂和聚合时间对AMP-MIP吸附性能的影响,通过扫描电镜、水中悬浮实验和吸附实验对AMP-MIP的形貌和性能进行了表征,得到最佳工艺条件:洗脱方式为超声、洗脱溶剂为甲醇-乙酸(4∶1,体积比)、聚合时间为30min。分子印迹聚合物不仅对小分子模板有吸附作用,而且对大分子ADP-核糖基化的甘油醛-3-磷酸脱氢酶(gapA)具有识别作用,吸附量可达3.9μg·mg^(-1)。Using polystyrene microsphere （CP） as a core, adenosine-5＇-monophosphate（AMP） as a template, aerylamide（AM） as a functional monomer and ethylene glycol dimethaerylate（EGDMA） as a eross-linker,while dimethylsulfoxide（DMSO） was chosen as a porogen and azobisisobutyronitrile（AIBN） as an initiator,core-shell adenosine-5＇-monophosphate molecular imprinted polymer（AMP-MIP） was prepared. The effects of different e- lution methods,solvents and polymerization time on the properties of AMP-MIP reeognition were discussed. The morphology and property of AMP-MIP were characterized by SEM,the suspension in aqueous medium and the binding experiments. The optimal process conditions were obtained as follows：the elution method was ultrasound,the elution solvent was methanol-acetic acid （4： 1） and polymerization time was 30 min. AMP-MIP showed good selectivities not only for AMP but also for glyceraldehyde-3-phosphate dehydrogenase （gapA） which was ADP-ribosylated protein. The adsorption capacity of gapA could reach 3.9μg· mg^-1.

关 键 词：聚苯乙烯 单磷酸腺苷 核壳聚合 分子印迹技术 甘油醛-3-磷酸脱氢酶 

分 类 号：TQ317[化学工程—高聚物工业]