利用DNA条形码技术发现贵州省蚋类新记录  

A New Record of Simuliidae Found by DNA Barcoding in Guizhou Province

作　　者：张圣芳[1] 贾若[2] 杨曜铭 王毅[2] 寻慧[2] 陈汉彬[2] 杨明[2] ZHANG Shengfang JIA Ruo YANG Yaoming Wang Yi XUN Hui CHEN Hanbin YANG Ming(Key Laboratory of Molecular Biology, Guizhou Medical University, Guiyang 550004, Guizhou , China Basic Medical College, Guizhou Medical University, Guiyang 550004, Guihzou, China)

机构地区：[1]贵州医科大学分子生物学重点实验室,贵州贵阳550004 [2]贵州医科大学基础医学院,贵州贵阳550004

出　　处：《贵州医科大学学报》2016年第11期1245-1248,共4页Journal of Guizhou Medical University

基　　金：贵州省社会发展攻关项目[黔科合SY(2012)3083];国家自然科学基金(30660021)

摘　　要：目的：综合利用DNA条形码序列和形态学特征鉴定蚋种。方法：利用车载式捕虫网于2014年7-9月在贵州省雷山、江口、德江、关岭和开阳5县采集蚋类标本，并进行初步的形态学鉴定；测定雌蚋DNA条形码序列，利用生命数据条形码系统（ BOLD ）及系统发育分析方法鉴定蚋种。结果：初步确定划分蚋属下蚋种的K2P距离阈值为4%，利用该阈值及生命数据条形码系统鉴定153只雌蚋为麻子绳蚋。结论：麻子绳蚋为贵州省蚋类新记录，分布于雷山、江口、德江、关岭和开阳5县。Objective：To identify blackfly species with combined data of DNA barcodes and morpho-logical characteristics. Methods：Blackflies were collected with a vehicle-mounted trap on July to Sep-tember of 2014 at Leishan,Jiangkou,Dejiang,Guanling,and Kaiyang counties in Guizhou Province, and preliminary morphological identification was performed. Female simulium DNA barcode sequence was detected. Barcode of Life Data Systems（ BOLD）and phylogenetic analysis methods were adopted to identify blackfly species. Results：It was preliminarily determined that the threshold value of K2P distance in differentiating Simulium blackfly species and 153 Simulium（ Gomphostilbia）asakoae indi-viduals were identified with BOLD and the threshold value. Conclusion：S. （ G. ）asakoae is a new record of blackfly,distributing at Leishan,Jiangkou,Dejiang,Guanling,and Kaiyang in Guizhou Province.

关 键 词：蚋科 DNA条形码 生物形态 贵州 生命数据条形码系统 系统发育分析 

分 类 号：R384.5[医药卫生—医学寄生虫学]