基于HPLC-Q-TOF-MS/MS技术的银黄清肺胶囊体外物质基础研究  

Study on substance basis of Yinhuang Qingfei Capsule in vitro by HPLC-Q-TOF-MS/MS

作　　者：周卿意骏 卿志星[1] 蔡萍[2] 肖娟[2] 高尚 张水寒[1,2,4] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省中医药研究院中药研究所,湖南长沙410013 [3]湖南安邦制药有限公司,湖南长沙410083 [4]湖南省2011数字中医药协同创新中心,湖南长沙410208

出　　处：《中草药》2016年第20期3586-3593,共8页Chinese Traditional and Herbal Drugs

基　　金：长沙市生物与新医药产业科技支撑重大专项项目:中药创新关键技术研究及产业化(K1204035-31)

摘　　要：目的采用高效液相色谱串联四级杆飞行时间质谱技术(HPLC-Q-TOF-MS/MS)对银黄清肺胶囊的化学成分进行检测和分析。方法采用Inertsil ODS-2 C_(18)色谱柱(250 mm×4.6 mm,5μm)反相HPLC梯度洗脱法分离各主要成分,电喷雾电离源经正离子模式对色谱流出物进行检测,四级杆飞行时间串联质谱法对各主要色谱峰进行归属;采用对照品、检索文献及各成分的图谱数据分析的方法分析化学成分。结果依据质谱裂解规律及文献检索,在正离子模式下,从银黄清肺胶囊样品中共鉴定出54个化合物。结论本实验建立了一种基于HPLC-Q-TOF-MS/MS技术对复方银黄清肺胶囊中化学成分进行鉴别的有效方法,为其质量控制及物质基础研究提供了技术支持。Objective To identify and analyze the chemical constituents in Yinhuang Qingfei Capsule by HPLC-Q-TOF-MS/MS method. Methods The HPLC method was used with the conditions that the column was Inertsil ODS-2 C18 （250mm×4.6mm,5μm）. Columu and electrospray ion （ESI） source was employed for the qualitative analysis under positive ion mode. These components were further analyzed by MS spectra, and by comparing with the corresponding reference substances and literature data. Results According to the MS principle and literature data, 54 compounds were identified from the sample ofYinhuang Qingfei Capsule. Conclusion An efficient HPLC-Q-TOF-MS/MS approach has been established for studying the chemical constituents in Yinhuang Qingfei Capsule, which paves a way for the quality control and further substance basis studies of the preparation.

关 键 词：银黄清肺胶囊 HPLC-Q-TOF-MS/MS麻黄碱 苦杏仁苷 贝母素甲 槲皮素 橙皮苷 

分 类 号：R284.1[医药卫生—中药学]