基于抗炎解热药效的小儿温热口服液中心复合设计-效应面法提取工艺优化研究  

Optimization of extraction process of Xiaoer Wenre Oral Liquid based on analgesic and anti-inflammatory pharmacodynamics by central composite design-response surface method

作　　者：余姣[1] 陈娇[1] 周文杰[2] 杨荣平[1,3] 

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室-省部共建国家重点实验室培育基地,四川成都611137 [2]天圣制药集团股份有限公司,重庆400065 [3]西南大学,重庆400715

出　　处：《中草药》2016年第20期3632-3638,共7页Chinese Traditional and Herbal Drugs

基　　金：基金项目:重庆市"121"科技支撑示范工程(cstc2016fazktjcsfl0009)

摘　　要：目的确立小儿温热口服液(XWOL)的最优提取工艺。方法通过镇痛、抗炎、解热药效学实验初选提取工艺路线,再以挥发油的提取量为指标,单因素考察浸泡时间、提取时间对鱼腥草等药材挥发油提取的影响;以连翘苷、黄芩苷的提取率及总评"归一值"为指标,通过中心复合设计-效应面法优选水提工艺。结果XWOL最佳提取工艺路线为鱼腥草等药材加入10倍量的水,水蒸气蒸馏6 h,收集挥发油及水煎液,药渣与黄芩等药材加入13倍量的水提取2次,每次80 min。结论该提取工艺合理,稳定,可用于工业化大生产。Objective To optimize the extraction process of Xiaoer Wenre Oral Liquid （XWOL）. Methods Through the analgesic, anti-inflammatory and antipyretic pharmacological experiments, the best extraction process route was primarily optimized, then taking the volatile oil amount as index, single factor test was adopted to investigate the effects of the soaking time and extracting time on extraction process of volatile oil; With contents of phillyrin, baicalin, and overall desirability as comprehensive evaluation indexes, central composite design-response surface method was adopted to predict the water extraction process. Results Adding 10 times water, extracting for 6 h, collecting volatile oil and water extract, then the dregs and other medicines were extracted twice with 13 times water, for 80 min each time. Conclusion The optimized extraction process is reasonable and feasible, and it can be used in a large scale industrial production.

关 键 词：小儿温热口服液 解热 抗炎 镇痛 连翘苷 黄芩苷 挥发油 中心复合设计-效应面法 

分 类 号：R284.2[医药卫生—中药学]