VGX-1027对光滑念珠菌刺激大鼠气道上皮细胞IL-1β和TNF-α表达影响的研究  被引量:1

Study of effect of VGX-1027 on expression of IL-1β and TNF-αin rat tracheal epithelial cells stimulated by Candida glabrata

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作  者:王玉春 张雪 骆雪萍 

机构地区:[1]桂林医学院第二附属医院检验科 [2]桂林医学院第二附属医院重症医学科,广西桂林541199

出  处:《重庆医学》2016年第32期4475-4478,共4页Chongqing medicine

基  金:广西医疗卫生重点科研课题(重2012007);广西自然科学基金项目(2013GXNSFAA019209)

摘  要:目的探讨未加免疫抑制剂VGX-1027大鼠气道上皮细胞(RTEC)与加入VGX-1027的RTEC分别与光滑念珠菌孵育后炎症因子IL-1β和TNF-α表达及意义。方法以体外培养未加VGX-1027孵育的RTEC(未加组)和加入VGX-1027孵育的RTEC(加入组),分别与光滑念珠菌孵育3、6、9h,观察未加和加入组RTEC形态变化;Real time PCR检测未加和加入组IL-1β和TNF-αmRNA表达;ELISA检测未加和加入组IL-1β和TNF-α分泌。未与光滑念珠菌孵育并未加VGX-1027的RTEC为对照组。结果随孵育时间延长,IL-1β和TNF-α表达呈进行性增高,未加组与加入组相比,未加组IL-1β和TNF-α表达更高。相同时间点,未加组与加入组比较IL-1β和TNF-α表达均差异有统计学意义(P<0.05)。结论 RTEC可识别光滑念珠菌启动天然免疫,并激活下游炎症因子IL-1β和TNF-α的分泌,介导炎症反应,但是过度的炎症反应不能起到保护作用反而加重损伤。Objective To explore the expression and. significance of IL-1β and TNF-α in rat tracheal epithelial ceUs(RTEC) without adding immunosuppressant VGX-1027 and RTEC with adding VGX-1027 after co-cuhuring with Candida glabrata. Methods: RTEC withoutt and with VGX-1027. were respectively: co-cultured With Candida glabrata bacteria liquid for 3,6,9h. Then the morphological changes of RTEC were observed in the adding VGX-1027 group and non-adding VGX-1027 group ; the mRNA ex- pressions of IL-1β and TNF-α were detected by real-time PCR and secretion of IL-1β and TNF-α was determined by ELISA. Results With the culture time extendlng,the expression of IL-1β and TNF-α showed the progressive increase^the expressions of IL-1β and TNF-α in the non-adding VGX-1027 group were higher than those in the adding VGX-1027 group. At the same time point,the ex- pressions of IL-1β and TNF-α had statlstically significant difference between the non-adding VGX-1027 group and adding VGX- 1027 group(P〈0.05). Conclusion RTEC can recognize Candida glabrata to activate natural immunity and secretion of downstream inflammatory factors IL-1β and TNF-α ,and mediates inflammatory reaction, but excessive inflammation can not play a protective effecton the contrary aggravates damage.

关 键 词:光滑念珠菌 大鼠气道上皮细胞 VGX-1027 肿瘤坏死因子α 白细胞介素1Β 

分 类 号:R459.7[医药卫生—急诊医学]

 

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