基于microRNAs调控的轻度慢性乙型肝炎炎症活动的分子机制研究  

Study on molecular mechanism of mild chronic hepatitis inflammatory activity based on microRNAs regulation

作　　者：张传涛[1,2] 黄群[2] 刘业方 郭尹玲[2] 辜海英[1] 王芳瑜[2] 胡蓉[3] 扈晓宇[1] 郑政隆[2] 

机构地区：[1]成都中医药大学附属医院感染科,成都610072 [2]成都中医药大学,成都610075 [3]成都市传染病医院肝病科,成都610066

出　　处：《重庆医学》2016年第32期4527-4530,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(81202624)

摘　　要：目的研究从microRNAs角度揭示轻度慢性乙型肝炎炎症活动的分子机制。方法将符合标准的病例分为轻度慢性乙型肝炎组与慢性HBV携带者组,借助Agilent Human microRNA 8×60k微阵列芯片检测血浆中microRNAs表达谱,求得两组间的差异表达microRNAs谱(P<0.05),借助microRNAs生物信息学分析软件预测其靶基因并对靶基因进行GO功能富集分析和pathway分析。结果两组间的差异表达microRNAs共65条(P<0.05),38条上调,27条下调;GO分析及pathway分析得到其功能主要涉及细胞增殖、生物黏附、生物合成过程的正/负调控、大分子生物合成过程中的正/负调控、蛋白氨基酸的磷酸化、RNA的生物合成过程、Wnt信号通路、MAPK信号通路、Notch信号传导途径、Hedgehog信号通路、T细胞受体信号通路、TGF-β信号通路、mTOR信号通路、趋化因子信号通路JAK-STAT信号通路、钙离子信号通路等。结论轻度慢性乙型肝炎炎症活动受特异性microRNAs调控,其涉及多个生命过程及通路。Objective To reveal the molecular mechanism of inflammatory activity of mild chronic hepatitis B from the per- spective of microRNAs regulation. Methods The cases conforming to the standard were divided into the mild chronic hepatitis B group and chronic HBV carriers group. The Agilent Human miRNA 8 × 60 k microarray chip was used to detect the expression pro- files of microRNAs in plasma in order to achieve different expression spectrum of microRNAs between the two groups （P〈0.05）. Then the miRNA bioinformatics analysis software was used to predict the target gene, and did target genes GO functional enrich- ment analysis and pathway analysis. Results A total of 65 microRNAs bands were differentially expressed between the two groups （P〈0.05）, 38 bands were up-regulated, 27 bands were down-regulated. The GO analysis and Pathway analysis showed that the function mainly involved in ceil proliferation, bioadhesion, positive/negative regulation of biosynthesis, positive/negative regulation of macromolecular biosynthesis,phosphorylated of protein amino acids,biosynthesis of RNA,Wnt. signaling, pathway, MAPK signa- ling pathway, Notch signaling transduction pathway, Hedgehog signaling pathway,T cell receptor signaling pathway, TGF-9 signa- ling pathway, roTOR signaling pathway, chemokine signaling pathway, JAK-STAT signaling pathway, calcium ion signaling path- way, etc. Conclusion The inflammatory activity of mild chronic hepatitis B is regulated by specific microRNAs, involving multiple life processes and pathways.

关 键 词：轻度慢性乙型肝炎 炎症活动 microRNAs调控 

分 类 号：R512.62[医药卫生—内科学]