利用EST-SSR技术分析银合欢种质资源遗传多样性  

Analysis of Genetic Diversity in Leucaena Germplasm Using Stylosanthes EST-SSRs

作　　者：邵辰光[1] 丁西朋 白昌军[2] 毕玉芬[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737

出　　处：《云南农业大学学报（自然科学版）》2016年第6期1031-1037,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金："十二五"国家科技支撑计划项目子课题(2011BAD17B03)

摘　　要：银合欢（Leucaena spp.）是豆科含羞草亚科银合欢属多年生木本植物,为了了解其种质资源的遗传多样性,采用184对引物对银合欢种质进行扩增,选择多态性较好的20对引物对48份银合欢种质进行遗传多样性分析。结果表明：184对引物中有119对为有效引物,占总引物数的64.67%,其中具有多态性的有效引物55对,占有效引物数的46.22%,占总引物数的29.89%;使用多态性较好的20对引物对48份银合欢种质进行遗传多样性分析,其相似系数为0.6182-0.9412,平均多态性信息指数和Shannon信息指数分别为0.266和0.536。通过聚类分析将48份种质在相似系数0.78处分为5个类群,全部异叶银合欢（Leucaena diversifolia）种质聚在了类群I并与银合欢种质分开;单独聚类在类群II的c27号种质与其他银合欢种质差异较大,是较好的种内杂交育种的种质;类群V中不同来源地的银合欢各种质聚在一起,可能与银合欢自交不育和育种方式有关。In order to analyze the genetic diversity of 48 Leucaena germplasm resources, 20 more polymor-phic effective markers were screened from 184 Stylosanthes EST-SSR markers. The result showed that 119 of 184 Styloanthes EST-SSR markers were effective markers and 55 markers were polymorphic, the effective and polymorphic transferable rate was 64. 67% and 29. 89%, respectively. Furthermore, in the genetic diversity analysis and cluster analysis of 48 Leucaena germplasm resources, the range of GS is 0. 618 2 0. 941 2, and the average of PIC and Shannon index are 0. 266 and 0. 536, respectively. All accessions were divided into 5 major group （I, II, III, IV and V）. All of the Leucaena diversifolia germplasm were clus- tered in Group I, and Leucaena leucaephala accessions from different sites were divided into Group V. Ac- cession e27 clustered separately in Group II is a good material for crossbreeding in Leucaena. In summary,the Stylosanthes EST-SSR markers can be utilized in analysis of genetic diversity, mining and identification of gene and breeding in Leucaena. Due to the complex genetic relationships, analysis of genetic relation- ships of Leucaena germplasm should be researched generally with several tools.

关 键 词：银合欢 柱花草 ESTSSRs 分子标记转移 

分 类 号：S541.903.3[农业科学—作物学]