彝族“黄药”(艾纳香)二氯甲烷提取物对Lewis肺癌小鼠抑瘤作用的研究  

Study on Tumor Inhibition of Dichloromethane Extract from Huang Yao (Blumea balsamifera DC.) of Yi Nationality in Lewis Lung Cancer Mice

作　　者：黄火强[1] 蒋慧嫦 张亚琛[1] 李杰[1] 

机构地区：[1]中央民族大学中国少数民族传统医学研究院,中国少数民族传统医学国家民委-教育部重点实验室,北京100081

出　　处：《云南农业大学学报（自然科学版）》2016年第6期1058-1064,共7页Journal of Yunnan Agricultural University:Natural Science

基　　金：国家自然科学基金项目(81303308)

摘　　要：彝族"黄药"(艾纳香)在少数民族地区被广泛用于治疗肺癌,前期研究发现"黄药"二氯甲烷提取物对A549细胞具有较好的抗癌细胞活性,因此,本试验在前期基础上,探讨彝药"黄药"二氯甲烷提取物对Lewis肺癌模型小鼠的抑瘤作用。通过建立小鼠Lewis肺癌模型观察"黄药"二氯甲烷提取物的抑瘤效应,采用H.E.染色法研究肿瘤组织病理变化,并运用免疫组化(IHC-P)法检测肺瘤组织中VEGF和TNF-α蛋白的表达情况。结果表明:同模型组相比,"黄药"二氯甲烷提取物能显著抑制小鼠Lewis肺癌的生长(P<0.05或P<0.01);经H.E.染色后,与模型组相比给药组肿瘤组织坏死明显,细胞密度降低,细胞核固缩、碎裂严重,其中高剂量组最为明显;免疫组化可检测到VEGF和TNF-α阳性表达存在于肿瘤细胞浆内,各给药组VEGF和TNF-α平均光密度值较模型组相比差异具有显著性(P<0.05)。可见,彝族"黄药"二氯甲烷提取物抑制小鼠Lewis肺癌作用明显,而且其抑瘤作用可能与下调VEGF和TNF-α蛋白的表达有关。Huang Yao （ Blumea balsamifera DC. ） of Yi nationality is widely used for treatment of lung cancer in minority areas, and the early research found that the dichloromethane extract from it has better anti-tumor activity on A549 cells. Therefore, on the basis of preliminary studies, we tried to study the anti-tumoreffect of the dichloromethane extract from Huang Yao of Yi nationality on Lewis lung cancer in mice. Through the establishment of model of C57BL/6J mouse with Lewis lung cancer, we observed the inhibitory effect of the dichloromethane extract from Huang Yao ; and used H. E. staining method to study the pathological changes in the tumor tissue; and applied the immunohistochemistry （IHC-P） method to detect the expression of VEGF and TNF-α protein in tumor tissues. The results showed that compared with the model group, the dichloromethane extract from Huang Yao could significantly inhibit the growth of Lewis lung carcinoma in mice （ P 〈 0. 05 or P 〈 0. 01 ）. The result of H. E. staining showed that the tumor tissue necrosis was obvious, the cell density was decreased, and the nuclear condensation and fragmentation was severe in the treatment group compared with the model group, which high dose group is the most obvious ; the positive expression of VEGF and TNF-α was detected by IHC-P in the cytoplasm of tumor cells, and the average optical density of VEGF and TNF-α in each group was significantly higher than that in the model group （P 〈 0. 05 ）. The experiment showed that the inhibition in mice with Lewis lung cancer was significant of the dichloromethane extracts from Huang Yao of Yi nationality, and the anti-tumor effect may be associated with the down-reg-ulation of the expression of VEGF and TNF-α protein.

关 键 词：艾纳香 LEWIS肺癌 抑瘤作用 

分 类 号：R733.6[医药卫生—肿瘤] S567.239[医药卫生—临床医学]