地被银桦试管苗增殖培养研究  

Proliferation of Grevillea ‘Billy Bonkers' via Tissue Culture

作　　者：李湘阳[1] 曾炳山[1] 裘珍飞[1] 

机构地区：[1]中国林科院热带林业研究所,广东广州510520

出　　处：《热带农业科学》2016年第10期28-32,共5页Chinese Journal of Tropical Agriculture

基　　金：948引进项目"地被银桦新品种及繁育技术引进"(No.2012-4-63)

摘　　要：地被银桦是杂交品种,无性繁殖对保持地被银桦的品种特性至关重要。本研究以组织培养为繁殖方法,从激素的浓度、种类、大量元素及糖等方面对地被银桦的增殖培养进行了系统的研究。结果表明,6-BA的浓度对增殖苗的高度有显著影响,6-BA和IBA对试管苗的玻璃化比例均有显著影响,NH_4NO_3和KH_2PO_4的浓度也显著影响增殖苗的高度。最适增殖培养基配方为:改良MS培养基(NH_4NO_3412.5 mg/L,KH_2PO_485 mg/L)+6-BA 0.3 mg/L+IBA 0.1 mg/L+糖35 g/L。Grevillea ＇Billy Bonkers＇ is a hybrid Grevillea. Vegetative propagation plays an important role in keeping varietal characteristics of ＇Billy Bonkers＇. ＇Billy Bonkers＇ was mass proliferated via tissue culture, and the hormones and their concentrations, macro-elements and sugar were modified to develop an optimal MS medium for tissue culture of ＇Billy Bonkers＇. The results indicated that the concentrations of 6BA, NH_4NO_3 and KH_2PO_4had significant effect on plantlet height growth of ＇Billy Bonkers＇. The 6BA and IBA had significant effect on plantlet vitrification ratio. The optimal medium for tissue culture is（NH_4NO_3412.5 mg/L, KH_2PO_485 mg/L） ＋ 6BA 0.3 mg/L ＋ IBA 0.1 mg/L ＋ sugar 35 g/L.

关 键 词：地被银桦 组织培养 增殖 

分 类 号：Q813.1[生物学—生物工程]