不同提取工艺蛋黄油对小鼠烫伤治疗作用研究  

Different Extraction Technology of Egg Butter in the treatment of Scalded Mice

作　　者：林影影 邹俊波[1] 何宜航 阳向波 纪奇森 韩丽[1] 张帅杰 

机构地区：[1]成都中医药大学中药材标准化教育部重点实验室四川省中药资源系统研究与开发利用重点实验室--省部共建国家重点实验室培育基地,四川成都611137 [2]四川联成迅康医药股份有限公司,四川成都610000 [3]成都百草和济科技有限公司,四川成都610000

出　　处：《亚太传统医药》2016年第22期12-15,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:考察不同提取工艺制备的蛋黄油对小鼠浅II度烫伤的治疗作用。方法:将240只SPF级ICR雌性小鼠按体重随机分为模型组、空白组、超临界CO_2萃取蛋黄油组、乙醇提取蛋黄油组、超临界CO_2+乙醇提取蛋黄油组和干馏蛋黄油组各40只,采用热砝码复制小鼠浅II度烫伤模型,连续治疗15天,每隔5天测定创面积,计算创面愈合率,实验结束后测定各组动物血清TGF-β1、IL-6水平。结果:与模型组比较,第5天所有受试组动物创面愈合率均显著提高(P<0.01),第10天样品3、4组创面愈合率明显提高(P<0.05),第15天样品1组仍明显提高创面愈合率作用(P<0.05);与模型组比较,样品2组第5天TGF-β1水平显著下降(P<0.01);第15天时,与模型组比较,样品1组TGF-β1水平显著降低(P<0.05);与模型组相比,给药第5天,样品2、3组实验动物IL-6水平明显下降(P<0.05);第10天时,各受试药物IL-6水平明显下降(P<0.05);第15天时,各受试物组IL-6水平呈下降趋势,但无统计学差异(P>0.05)。结论:不同提取工艺所得蛋黄油均对小鼠浅Ⅱ度烫伤模型具有一定的治疗作用。Objective：To investigate the preparation of different processing methods of egg yolk oil for shallow II mouse burns treatment. Methods： 240 SPF grade female ICR mice were randomly divided into six groups： model group, the control group, yolk oil supercritical extraction group, ethanol extract of egg yolk oil group, supercritieal ＋ ethanol extract group and the yolk oil distil- lation group. Copy the shallow thermal weights burns Ⅱ mouse model of continuous treatment 15d, measuring the wound area every 5 days, to calculate the rate of wound healing, end of the experiment measured the levels of serum TGF-β1, IL-6. Results：Compared with the model group, the first five days all animals tested healing rate increased significantly （P〈0.01）, the first 10 days 3, 4 sample group significantly increased the rate of wound healing （P〈0.05）, 15th day 1 sample group has significantly increased the rate of wound healing effects （P〈0.05） ; compared with the model group, sample 2 group 5 days TGF-β1 levels decreased significantly （P〈0.01） ; the first 15 days, with the model group ratio, sample 1 group of TGF-β1 levels were significantly lower （P〈 0.05） ; compared with the model group, the administration of the first five days, a sample group of experimental animals 2,3 IL-6 levels were significantly decreased （P〈0.05, P〈0.01） ; the first 10 days of each test drug IL-6 levels were significantly decreased （P〈0.05） ; the first 15 days of each test substance group IL-6 levels decreased, but there was no statistically significant difference （P〉0. 05）. Conclusion： Eggs and butter are from different extraction technology of shallow Ⅱ degree scald in mice model has a certain therapeutic effect.

关 键 词：蛋黄油 超临界萃取 浅Ⅱ度烫伤 

分 类 号：R285.5[医药卫生—中药学]