白屈菜红碱预处理对糖尿病大鼠肾缺血再灌注远程心肌损伤的保护作用研究  

Protective Effects by Chelerythrine Pretreatment on Renal Ischemia/Reperfusion Myocardium Injury in Diabetic Rats

作　　者：徐志晓 潘晓燕[1] 杨婷婷[1] 扬翼 陈场[1] 应羽洲 Xu Zhixiao Yang Tingting Yang Yi Chen Chang Ying Yuzhou Pan Xiaoyan(College of Medicine, Jiaxing University, Jiaxing, Zhejiang 314001)

机构地区：[1]嘉兴学院医学院,浙江嘉兴314001

出　　处：《嘉兴学院学报》2016年第6期64-68,共5页Journal of Jiaxing University

基　　金：浙江省大学生科技创新活动计划暨新苗人才计划项目(2015R417002);浙江省实验动物科技计划项目(2015C37130)

摘　　要：观察白屈菜红碱预处理对糖尿病大鼠肾缺血再灌注心肌损伤的影响,并探讨其可能的作用机制.成年SD大鼠分为正常组、糖尿病组、缺血再灌注组和白屈菜红碱预处理+缺血再灌注组,各组动物经1%戊巴比妥钠腹腔麻醉,开腹,暴露双侧肾动、静脉,各组经缺血再灌注或经白屈菜红碱预处理后检测相应试验指标.结果显示糖尿病大鼠肾脏缺血再灌注后,I/R组肾功能明显下降,BUN和Scr均明显高于sham和DM组,心肌组织SOD活力较sham和DM组明显降低,LDH、CK活力和MDA含量显著升高.与I/R组相比,白屈菜红碱组BUN、Scr显著减少,SOD活力明显增加,LDH、CK活力和MDA含量明显降低.白屈菜红碱组心肌组织NF-κB表达降低.得出结论,缺血再灌注后,肾功能和心功能明显受损.白屈菜红碱预处理可以减轻糖尿病大鼠肾缺血再灌注远程心肌损伤,改善心肌功能.This papers aims to observe the effects with Chelerythrine pretreatment on renal ischemia/ reperfusion myocardium injury in diabetic rats and explore its possible mechanism. Adult SD rats are divided into normal group, diabetes mellitus group, ischemia reperfusion group and Chelerythrine pretreatment＋ ischemia reperfusion group. Rats in each group are opened and their bilateral renal arteries and veins are exposed after in- traperitoneal anesthesia by 1% of Pelltobarbitalum Natricum and the relevant experiment indexes are tested in each group after ischemia/reperfusion or/and Chelerythrine pretreatment. The results show that after renal ischemia/reperfusion in diabetic rats, renal function of I/R group decreases in a significant way, BUN and Scr are much higher than those of sham and DM groups; the activity of SOD in myocardial tissue decreases to a greater extent than that of sham and DM group; the activity of LDH and CK and the content of MDA increases noticeably. Comparing with I/R group, BUN and Scr in chelerythrine＋DM＋ I/R group significantly reduces, but the activity of SOD increases obviously, and the activity of LDH and CK and the content of MDA decrease to a greater degree; and the expression of NF-kB in the myocardium decreases. In conclusion, after ischemia/ reperfusion, renal function and cardiac function are impaired; Chelerythrine pretreatment can reduce myocardial injury after renal ischemia reperfusion which causes distance injury in diabetic rats, and improve myocardial function.

关 键 词：肾缺血再灌注 心肌损伤 白屈菜红碱预处理 NF-ΚB 

分 类 号：R692.02[医药卫生—泌尿科学]