烫伤宁制剂质量标准的研究  

A Study on Quality of Tangshangning Preparation

作　　者：叶绘晟[1] 柳贤福[1] 龚志强[1] Ye Huisheng Liu Xianfu Gong Zhiqiang(Faculty of Chinese Medical Science Guangxi University of Chinese Medicine, Guangxi, 53022)

机构地区：[1]广西中医药大学赛恩斯新医药学院,广西530222

出　　处：《当代化工研究》2016年第8期62-63,共2页Modern Chemical Research

摘　　要：建立烫伤宁制剂的质量控制标准。方法：薄层色谱法对烫伤宁制剂中的虎进行定性鉴别;采用高效液相色谱方法测定虎杖中大黄素含量。色谱柱：Hypersil ODS C18柱（250mm×4.6mm,5μm）,流动相为甲醇-0.2%磷酸溶液（85：15）,检测波长为254nm,流速1.0m L·min^-1,柱温30℃。结果：薄层色谱斑点清晰,阴性对照无干扰;大黄素进样量在0.14-2.24μg之间呈良好的线性关系（r=0.9999）,平均加样回收率为99.73%,RSD为1.59%（n=9）。结论：所建方法简单、稳定、重复性好,可用于烫伤宁制剂的质量控制。OBJECTIVE： To establish the standard for the quality control of Tangshangning Preparation. Method： The identification of Polygonum cuspidatum was carried out by TLC. The content of emodin in Polygonum cuspidatum by HPLC. The samples were separated on the Hypersil ODS C18（250mm ×4.6mm,5μm） colunm which was eluted with methanol-0.2% phosphoric acid solution（85：15） with detective wavelength at 254 nm and flow rate at 1m L·min^-1,column temperature at 30℃. Results： The TLC spots were highly clear without the interference of negative control and were reproductive. The method had good linear relationship within the range of 0.14-2.24μg（r=0.9999）for quercetin. The average recovery rate was 99.73% with RSD of 1.59%. Conclusion： The method is simple,rapid and reliable which can be used for the quality control of the Tangshangning Preparation.

关 键 词：烫伤宁 虎杖 大黄素 薄层色谱法 高效液相色谱法 

分 类 号：TQ461[化学工程—制药化工]