抗TNF-α与透明质酸共轭结合对抑制烧伤炎症反应的效果  

Study on the inhibitory effect of anti TNF-alpha and hyaluronic acid conjugate on the inflammatory response in burn

作　　者：胡强[1] 隋爽[1] 王国栋[1] 姚利民[1] 

机构地区：[1]山东省烟台市解放军第107医院烧伤整形科,山东烟台264000

出　　处：《中国生化药物杂志》2016年第10期33-36,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的制备抗TNF-α与透明质酸(hyaluronic acid,HA)轭合物,研究其对烧伤炎症反应的抑制效果。方法制备了抗TNF-α抗体与HA共轭结合物,并将其烧伤抗炎效果与生理盐水、HA、抗TNF-α抗体以及HΑ与抗TNF-α非共轭混合物进行比较研究。结果该轭合物会减少无法存活的组织,并使巨噬细胞CD68减少,IL-1β浓度降显著降低。结论研究结果表明,与直接运用非共轭抗体比较,抗TNF-α与高分子量HΑ共轭结合应用能更持久的局部调节损伤后炎症反应。Objective To study the inhibitory effect of anti TNF-alpha and hyaluronic acid（ HA） conjugate on the inflammatory response in burn.Methods Anti TNF alpha antibody was prepared with HA conjugate,and compared the anti-inflammatory effects with HA,anti-TNF-α antibody and HΑwith anti-TNF-α unconjugated mixture. Results The conjugate can reduce the survival of the tissues,reduced the CD68 of macrophages and decreased the IL-1β concentration. Conclusion The results indicate that,compared with direct use of Non-conjugated antibody,the application of anti-TNF-αconjugated to high molecular weight HΑ can adjust the inflammatory response after injury more lasting.

关 键 词：TNF-Α 伤口愈合 透明质酸 烧伤创面 炎症 

分 类 号：R644[医药卫生—外科学]