XN2000血细胞分析仪2种模式检测低值血小板的比较  

Comparison on two modes of Sysmex XN2000 hematology analyzer

作　　者：邓俊[1] 向延根[1] 许靖[1] 吴小梅[1] 

机构地区：[1]湖南省长沙市中心医院检验科,410004

出　　处：《国际检验医学杂志》2016年第22期3132-3133,3136,共3页International Journal of Laboratory Medicine

摘　　要：目的通过分析和比较希森美康XN2000血细胞分析仪中的2种血小板检测通道,寻找合理的低值血小板检测模式。方法选取首次检测血小板计数小于或等于50×109/L的临床血常规标本72例,使用XN2000血细胞分析仪的电阻抗(PLT-I)通道和荧光染色(PLT-F)通道检测血小板;根据血涂片将标本分为血小板无聚集组和聚集组,对无聚集组结果采用配对t检验、线性回归和Bland-Altman法偏差分析进行统计学分析;聚集组结果与手工血小板计数结果比对。结果无聚集组2种通道的血小板检测结果相关性较大,偏差较小,配对t检验差异无统计学意义(P>0.05);而聚集组结果相差较大,且PLT-F结果更接近手工血小板计数结果。结论血小板无聚集时可只选择PLT-I通道检测,仪器有聚集报警时选择PLT-F通道较准确。Objective To seek the rational low value platelet detection mode by analyzing and comparing the two platelet detection channels of the Sysmex XN2000 Hematology Analyzer.Methods Seventy-two clinical blood routine specimens of the platelet≤50×10^9/L at the first time detection were selected and detected by impedance channel（PLT-I）and fluorescence staining channel（PLT-F）offered by the Sysmex XN2000 Hematology Analyzer.According to the blood smear,the specimens were divided into the non-aggregation group and aggregation group.Then the results of the non-aggregation group adopted the paired t test,linear regression and Bland-Altman bias analysis for conducting the statistical analysis;the detection results were compared between the aggregation group and the manual platelet count.Results The results of the two channels in the non-aggregation group had large correlation and less bias,the difference of the paired t test had no statistical significance;but the results of aggregation group had larger difference,moreover the PLT-F results were more close to the results of the manual platelet count.Conclusion When no platelet aggregation,the detection can select the PLT-I channel,but when the instrument has the alarm of aggregation,selecting the PLT-F channel will be more accurate.

关 键 词：血细胞分析仪 血小板计数 电阻抗 荧光染色 

分 类 号：R446.11[医药卫生—诊断学]