香烟烟雾染毒对雄性大鼠睾丸组织ATP酶活性的影响  

The effect of cigarette smoke exposure on ATPase enzyme activity in testis of male rats

作　　者：张静[1] 仲春雪 黄云飞[1] 张晨[1] 何丽娟[1] 邹莹[1] 

机构地区：[1]新疆医科大学公共卫生学院毒理学教研室,乌鲁木齐830011

出　　处：《天津医药》2016年第11期1343-1346,共4页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81260433);新疆维吾尔自治区自然科学基金资助项目(2014211C015)

摘　　要：目的探讨香烟烟雾染毒对大鼠睾丸结构及睾丸组织ATP酶活性的影响。方法清洁级成年雄性SD大鼠70只,随机分为对照组(10只)和低、中、高剂量染毒组(每组20只,分别给予10支、20支、30支香烟烟雾暴露)。染毒组采用呼吸道静式染毒,每日1次,每次30 min,各剂量染毒组分别染毒6周和12周(每个时段10只大鼠)。实验期间记录各组大鼠体质量变化。实验结束后,摘取睾丸,HE染色观察睾丸组织结构,酶联免疫吸附试验检测睾丸组织Na+-K+-ATP酶、Ca^(2+)-ATP酶活性。结果随染毒剂量增加,各组大鼠体质量逐渐降低(P<0.05)。染毒组大鼠睾丸病理切片观察到不同程度损伤,且随着染毒时间的延长和染毒剂量的增加,损伤逐渐加重。染毒6周后,高剂量组大鼠睾丸组织Na+-K+-ATP酶活性低于其他3组(P<0.05),Ca^(2+)-ATP酶活性低于对照组(P<0.05)。染毒12周后,低、中、高剂量组大鼠睾丸组织Na+-K+-ATP酶、Ca^(2+)-ATP酶活性均较对照组显著降低(P<0.05)。结论香烟烟雾染毒可减缓雄性大鼠生长,导致睾丸组织损伤,Na+-K+-ATP酶和Ca^(2+)-ATP酶活性降低。Objective To explore the effect of cigarette smoke exposure on testis structure and ATPase enzymeactivity in testis of rats. Methods Seventy adult male SD rats were randomly divided into the control group(10 rats) and thelow, medium, high- dose groups(20 rats in each group, which received the exposure of 10, 20, 30 sticks of cigarettesrespectively). The experimental group was exposed to cigarette smoke for 30 min per day. Each dose group was exposed forsix weeks and twelve weeks(10 rats each time). The body weights in each group were recorded during the experiment. At theend of the experiment, testes of rats were removed, and the testicular tissue structure was observed by HE staining. Na+-K+-ATPase enzyme and Ca^(2+)-ATPase enzyme activity of testicular tissue of rats were detected by enzyme-linked immunosorbentassay. Results With the increasing of exposure doses, the body weights of rats decreased(P < 0.05). The microstructurechanges of testis were observed in the experimental group, and with the increasing of exposure time and exposure dose, thedamage was gradually worse. After 6- week exposure, the Na+- K+- ATPase enzyme activity of testicular tissue wassignificantly lower in high dose group than that of rest of three groups(P < 0.05), and the Ca2 +-ATP enzyme activity wassignificantly lower than that of the control group(P < 0.05). The activities of Na+-K+-ATPase enzyme and Ca2 +-ATPaseenzyme in testicular tissue were significantly decreased in the low, medium and high-dose groups than those of control groupafter being exposed for 12 weeks(P < 0.05). Conclusion Cigarette smoke exposure can retard the weight gain, cause thetestis tissue damaged and decrease the activities of Na+-K+-ATPase enzyme and Ca^(2+)-ATPase enzyme in testis of male rats.

关 键 词：吸烟 烟草 烟雾 大鼠 Sprague-Dawley 睾丸 腺苷三磷酸酶类 钠钾交换ATP酶 钙转运ATP酶类 

分 类 号：R114[医药卫生—卫生毒理学]