热敏灸对脑缺血再灌注损伤大鼠大脑皮质CD11b表达的影响  

Effects of heat-sensitive moxibustion on the expression of CD11b in cortex of focal cerebral ischemia-reperfusion injury model rats

作　　者：龚丽丽[1] 张毫[1] 谢斌[1] 欧阳昕[1] 肖爱娇[1] 

机构地区：[1]江西中医药大学基础医学院,330004

出　　处：《天津医药》2016年第11期1351-1355,共5页Tianjin Medical Journal

基　　金：国家自然科学基金资助项目(81060305,81660819);江西省自然科学基金资助项目(20151BAB205068,20161BBH80053);江西省卫生计生委中医药科研计划项目(2014Z003);江西中医药大学课题(2014ZR018,2015JZZDXK024)

摘　　要：目的制备局灶性脑缺血性再灌注损伤大鼠模型,观察热敏灸对模型大鼠大脑皮质CD11b表达的影响。方法雄性SD大鼠51只,采用随机数字表法将大鼠分为假手术组(n=10)、模型组(n=14)、艾灸组(n=27)。艾灸组根据艾灸前后尾温变化再分为非热敏灸组(n=13)和热敏灸组(n=14)。应用线栓法制备局灶性脑缺血再灌注损伤大鼠模型。采用神经功能缺失评分法观察大鼠的行为学表现;光照测痛仪测定大鼠的甩尾潜伏期;TTC染色法观察大鼠的脑梗死面积;免疫组织化学法检测大脑皮质CD11b的表达。结果术后3 d时,非热敏灸组(9.32±1.11)s与热敏灸组(8.69±0.51)s大鼠甩尾潜伏期均明显短于模型组(12.21±1.04)s,以热敏灸组缩短更为明显;非热敏灸组(20.59±2.25)%和热敏灸组(13.18±3.50)%大鼠脑梗死面积小于模型组(32.22±14.20)%,以热敏灸组缩小更为明显;非热敏灸组(5.40±0.58)和热敏灸组(2.89±0.71)大鼠大脑皮质CD11b表达低于模型组(7.83±1.60),以热敏灸组降低更为明显。结论热敏灸具有改善大鼠的感觉功能、减轻大鼠脑缺血再灌注损伤的作用,减少大脑皮质CD11b的表达、减轻炎症反应可能是其作用机制之一。Objective To observe the effect of heat-sensitive moxibustion on the expression of CD11 b in focal cerebralischemia/reperfusion(I/R) injury model rats. Methods Fifty-one male SD rats were randomly divided into three groups,sham- operated group(n=10), I/R injury group(n=14) and moxibustion group(n=27). According to changes of tailtemperature before and after moxibustion, moxibustion group was subdivided into non heat-sensitive moxibustion group(n=13) and heat- sensitivel moxibustion group(n=14). Focal cerebral ischemia- reperfusion injury was induced by middlecerebral artery occlusion for 2 hours and reperfusion for 3 days. Behavioral performance was tested using neurologic deficitscores. Tail-flick latency was determined with tail flick analgesia meter. Infarct areas were examined using TTC staining,and cortical expression of CD11 b was measured using immunohistochemical staining. Results After 3 d of operation, the tail- flick latency was significantly shorter in non heat- sensitive moxibustion group(9.32 ± 1.11) s and heat- sensitive moxibustion group(8.69±0.51) s than that of model group(12.21±1.04) s. The area of cerebral infarction was smaller in non heat-sensitive moxibustion group(20.59±2.25)% and heat-sensitive moxibustion group(13.18±3.50)% than that in model group(32.22 ± 14.20)%. Compared with model group, the expression of CD11 b was significantly decreased in non heatsensitive moxibustion group and heat-sensitive moxibustion group(P<0.05). Conclusion Heat-sensitive moxibustion can reduce the damage of cerebral inchemia-reperfusion, which might be through decreasing expression of CD11 b.

关 键 词：艾炷灸 脑缺血 再灌注损伤 抗原 CD11B 大鼠 Sprague-Dawley 甩尾潜伏期 

分 类 号：R743[医药卫生—神经病学与精神病学]