布伦登卢普沙门菌耐药性与毒力基因分析  

Antimicrobial resistance and virulence gene profiling of Salmonella enterica serovars Braenderup

作　　者：赵展[1] 夏琳林 刘晓霞[1] 杨杰[1] 吴晓妹[1] 张利娟[1] 贾宇驰 王玉宝[1] 

机构地区：[1]天津医科大学第二医院感染性疾病研究所,300211

出　　处：《天津医药》2016年第11期1387-1390,共4页Tianjin Medical Journal

基　　金：天津市卫生局科研基金资助项目(2013KZ112)

摘　　要：目的：研究临床分离的非伤寒沙门菌布伦登卢普血清型（布伦登卢普沙门菌）对抗菌药物的耐药性及其携带的毒力基因特征。方法收集2012—2014年每年4—10月天津市两家教学医院肠道门诊急性腹泻患者的临床资料，将急性腹泻患者粪便标本进行沙门菌分离培养、生化及PCR鉴定、血清学分型，对得到的布伦登卢普沙门菌进行抗菌药物敏感性检测、PCR扩增沙门菌毒力岛（SPI）1-5的代表性基因和SPI的调节基因。结果3年共检测到非重复非伤寒沙门菌153株，其中8株（5.23%）为布伦登卢普沙门菌。8株invA-PCR鉴定均呈阳性，对萘啶酸耐药率100%，对环丙沙星和左氧氟沙星中介耐药率100%，对其余检测的抗菌药物敏感；8株均检测到了SPI 1-5代表性基因和SPI调节基因（sitC、hilA、sseL、sifA、mgtC、siiE、sopB和phoP）。结论天津地区布伦登卢普沙门菌临床株对氟喹诺酮耐药并携带SPI 1-5毒力基因与调节基因，对公众健康构成潜在威胁，应持续开展相关监测与研究。Objective To investigate antimicrobial resistance and virulence gene profiles of clinically isolated non-typhoidal Salmonella enterica serovars Braenderup(S. Braenderup). Methods Clinical date of patients with acute diarrheafrom two teaching hospitals in our city were collected from April to October of 2012 to 2014. Salmonella from stool sampleswas screened by culture, and identified by biochemical, PCR and serotype methods. Isolates of S. Braenderup were examinedwith antibiotic susceptibilities. PCR amplification was used for representative genes of Salmonella pathogenicity islands(SPI) 1-5 and SPI regulator. Results Among 153 non-repetitive NTS isolates in the three years, 8 isolates(5.23%) of S. Breanderup were identified. All 8 isolates were positive with inv A gene. The resistance rate to nalidixic acid was 100%,and the same with the intermediate resistance rate to ciprofloxacin and levofloxacin. But they were susceptible to otherantimicrobial agents. The 8 isolates were positive with the representative genes of SPI 1-5 and regulators(sit C, hil A, sse L,sif A, mgt C, sii E, sop B and pho P). Conclusion Clinical isolates of S. Braenderup in Tianjin are resistant to fluoroquinolonesand harbored genes of SPI 1-5 and regulator, and which can threaten public health. Continuous surveillance and researchshould be carried out in our city.

关 键 词：腹泻 抗药性 微生物 喹诺酮类 氟喹诺酮 毒力 布伦登卢普沙门菌 

分 类 号：R378.22[医药卫生—病原生物学]