舒肺贴对COPD大鼠肺组织IL-8、TNF-α及黏蛋白MUC5AC表达的影响  

Effects of Shufei Plaster on the expressions of IL-8,TNF-α and mucin MUC5AC in lung tissue of COPD rats

作　　者：周昌妮 韩德恩[1] 徐如冰[1] 朱泠音 申延利 贾永艳[1,2] 

机构地区：[1]河南中医药大学,河南郑州450046 [2]呼吸疾病诊疗与新药研发河南省协同创新中心,河南郑州450046

出　　处：《中成药》2016年第11期2319-2324,共6页Chinese Traditional Patent Medicine

基　　金：呼吸疾病诊疗与新药研发河南省协同创新中心基金项目(2013003);河南省科技攻关计划项目(092102310112);河南中医学院研究生创新基金项目(2014YCX003)

摘　　要：目的探讨舒肺贴(芫花、芥子、延胡索等)对慢性阻塞性肺疾病(COPD)大鼠血清、支气管肺泡灌洗液(BALF)中炎症因子水平,以及肺组织黏蛋白基因MUC5AC表达的影响。方法将80只健康SD雄性大鼠随机分为正常对照组、模型组、舒肺贴组、氨茶碱组。采用烟熏联合脂多糖(LPS)感染法建立大鼠慢阻肺模型,AniRes2005动物肺功能分析系统测定大鼠肺功能,ELISA法检测各组大鼠血清、BALF中IL-8、TNF-α含有量,免疫组织化学染色观察黏蛋白基因MUC5AC的表达。结果 ELISA检测结果显示,模型组、舒肺贴组和氨茶碱组大鼠BALF和血清中IL-8、TNF-α含有量明显高于正常对照组,舒肺贴组和氨茶碱组与模型组相比均显著降低。相比舒肺贴组,氨茶碱组在降低TNF-α含有量方面效果更显著,但两者在降低IL-8含有量方面效果无明显差异。免疫组化结果显示,黏蛋白基因MUC5AC主要表达于支气管管腔内,其次是上皮、间质组织,淋巴组织也有少量表达。模型组积分光密度IOD值较正常对照组升高,而舒肺贴组、氨茶碱组较模型组明显降低。结论舒肺贴可以明显改善慢阻肺大鼠的肺通气功能,降低炎症因子IL-8、TNF-α含有量,减轻气道及肺组织炎症,下调MUC5AC黏蛋白,对气道黏蛋白高分泌有明显的抑制作用。AIM To investigate the effects of Shufei Plaster（ Genkwa Flos,Sinapis Semen,Corydalis Rhizoma,etc.） on the levels of inflammatory cytokines in serum and bronchoalveolar lavage fluid（ BALF）,and the expression of mucin MUC5 AC in lung tissue of rats with chronic obstructive pulmonary disease（ COPD）. METHODS Eighty healthy male SD rats were randomly divided into control group,model group,Shufei Plaster group and aminophylline group. The rat model of chronic obstructive pulmonary disease was established by smoking combined with lipopolysaccharide（ LPS）. The pulmonary function of rats was detected by AniRes2005 animal lung function analytic system. The contents of IL-8 and TNF-α in rats’ serum and BALF were detected by ELISA method. The expression of mucin MUC5 AC was observed by immunohistochemical staining. RESULTS ELISA results showed that the contents of IL-8 and TNF-α in serum and BALF of rats in model,Shufei Plaster and amin-ophylline groups were significantly higher than those of the control group. These values in Shufei Plaster group and aminophylline group were significantly lower than those in the model group. Compared with Shufei Plaster group,the aminophylline group was more significant in reducing the TNF-α level. But between the two groups,there were no significant difference in reducing the IL-8 level. Immunohistochemistry results showed that mucin MUC5 AC was mainly expressed in the bronchial lumen,followed by the epithelial and mesenchymal tissues,and was also expressed a little in the lymphoid tissue. IOD value of the model group was significantly higher than that of the control group,but IOD values of Shufei Plaster and aminophylline groups were markedly lower than that of the model group. CONCLUSION Shufei Plaster can improve the pulmonary ventilation function in rats with chronic obstructive pulmonary disease,reduce the contents of inflammatory cytokines IL-8,TNF-α and the inflammation of airway and lung tissue,and decrease mucin MUC5 AC. It has an apparent inhibitory effect

关 键 词：舒肺贴 慢性阻塞性肺疾病(COPD) 炎症因子 黏蛋白基因MUC5AC 

分 类 号：R285.5[医药卫生—中药学]