SARA状态下瘤胃产生的LPS对奶牛乳腺组织细胞凋亡的影响  

Effect of lipopolysaccharide derived from the rumen on mammary tissue cell apoptosis in dairy cows suffering SARA

作　　者：严金玉[1] 刘欣欣[1] 代宏宇[1] 常广军[1] 张凯[1] 徐天乐[1] 金笛[1] 郭峻菲 沈向真[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095

出　　处：《畜牧与兽医》2016年第11期32-37,共6页Animal Husbandry & Veterinary Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2011CB100802);国家自然科学基金资助项目(31172371)

摘　　要：本试验采用12头泌乳中期的荷斯坦奶牛,随机分为两组,每组6只。其中一组饲喂低精料日粮,即日粮中含有40%的精料和60%的粗料,精粗比为4∶6,作为对照(LC)组;另外一组饲喂高精料日粮,精粗比为6∶4,作为试验(HC)组。试验周期为18周,试验开始前为所有奶牛安装瘤胃瘘管,试验后期采集瘤胃液,乳动脉、静脉血和乳腺组织样品,测定瘤胃液p H值和LPS含量、血浆中LPS含量、乳腺组织细胞中凋亡相关基因如caspase-3、caspase-8、caspase-9、bax和bcl-2等的表达、凋亡相关蛋白酶的活性和NF-κB通路中的蛋白表达。结果显示:长期高精料饲喂使奶牛瘤胃p H值均值降低,一天中p H值低于5.6的时间超过3 h;高精料组血浆和瘤胃液中LPS含量均极显著高于低精料组(P<0.01);高精料组caspase-3的基因表达显著高于低精料组(P<0.05),而bcl-2的基因表达显著低于低精料组(P<0.05);高精料组caspase-3和caspase-8的酶活性相比于低精料组都显著升高(P<0.01);高精料组NF-κB p65和磷酸化的NF-κB p65的蛋白表达量均显著高于低精料组(P<0.05)。Twelve Holstein dairy cows in middle lactation were used and allocated into two groups（ 6 per group） randomly. One group was fed with a diet composed of 60% forage and 40% concentrate（ low-concentrate,LC group） as a control,while the other group received a diet composed of 40% forage and 60% concentrate（ high-concentrate,HC group） as a treatment. The experiment period was 18 weeks. Rumen fistula was fit for all the cows before the experiment started and ruminal liquid,mammary artery and vein blood and mammary tissues were sampled at the end time of the trial to detect the ruminal p H value,LPS（ lipopolysaccharide） concentrate,the apoptosis-related gene such as caspase-3,caspase-8 and caspase-9 expression,the caspase-3 and caspase-8 enzyme activities and NF-κB protein content. The results showed that long-term high-concentrate diet feeding led to a low p H average value and being below 5. 6 for 3 hours. LPS concentrations in plasma of HC group were significantly（ P〈0. 01） higher than LC group. HC group got a significantly（ P〈 0. 05） higher caspase-3gene expression and remarkably（ P〈0. 05） lower bcl-2 gene expression than LC group. Caspase-3 and caspase-8 enzyme activities in HC group were significantly（ P〈0. 01） higher than those in LC group. NF-κB p65 and phospho-NF-κB p65 protein content in HC group were significantly（ P〈0. 05） higher than those in LC group.

关 键 词：高精料日粮 奶牛 乳腺组织细胞 凋亡 

分 类 号：S854[农业科学—临床兽医学]