针刺、艾灸三阴交穴对寒凝类痛经模型大鼠影响的对比研究  

The Comparative Study on Acupuncture with Moxibustion at “‘Sanyinjiao'(SP6)” on Dysmenorrheal Rats Model with Cold Syndrome

作　　者：崔晓[1] 周瑾[1] 杨佳敏[1] 郭孟玮[1,2] 赵雅芳[1,2] 张梅[1] 刘珍珍[1] 齐丹丹[1] 朱江[1] 任晓暄[1,2] 张露芬[1,2,3] 

机构地区：[1]北京中医药大学,北京100029 [2]国家中医药管理局针灸生物学三级实验室,北京100029 [3]国家中医药管理局针灸特色疗法评价重点研究室,北京100029

出　　处：《中医药导报》2016年第22期10-13,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)资助项目(2012CB518506);国家中医药管理局针灸特色疗法评价重点研究室

摘　　要：目的:观察针刺、艾灸三阴交穴对寒凝类痛经模型大鼠子宫张力和子宫收缩素受体(OTR)的影响,探讨不同方法干预三阴交穴对寒凝类痛经的效应差异及机制。方法:将处于动情间期的3月龄雌性SD大鼠,随机分为盐水组、模型组、针刺组和艾灸组,每组7只。除盐水组外,模型组、针刺组和艾灸组均采用全身冷冻法结合苯甲酸雌二醇注射法造模。盐水组和模型组不予干预,针刺组针刺三阴交穴;艾灸组予以常规方法艾灸三阴交穴。运用BL-420F生物机能实验系统记录仪记录子宫的收缩反应;采用RT-PCR方法检测大鼠子宫组织中缩宫素受体(OTR)m RNA的表达。结果:模型组大鼠子宫的收缩波个数、波峰峰值、子宫活动度及子宫组织中OTR m RNA表达均高于盐水组(P<0.01或P<0.05);针刺组、艾灸组大鼠子宫的收缩波个数以及子宫组织中OTR m RNA表达均明显低于模型组(P<0.01);艾灸组大鼠的子宫活动度低于模型组(P<0.05);艾灸组大鼠子宫组织中的OTR m RNA表达水平明显低于针刺组(P<0.01)。结论:针刺或艾灸三阴交穴均可有效缓解寒凝类痛经大鼠的疼痛,且艾灸的效应优于针刺。Objective： To investigate the differences and the reaction mechanism of different intervention methods on dysmenorrheal rats model with cold syndrome by needling alone or moxibustion at ＂＇Sanyinjiao＇ （SP6）＂, observing the tension of the uterus and the expression of contraction receptor （OTR） of uterine tissue in rats. Methods： The female SD rats at diestrus with 3 months aged were randomized into 4 groups, including a saline control group, a cold stagnation of dysmenorrheal model control group, an acupuncture group and a moxibustion group, with 7 rats in each group. Except the saline control group rats received intraperitoneal injection 0.9% NaCl, the rats in the rest groups were treated with the whole body freezing method combined with estradiol benzoate injection mold. Except the saline control group and model control group, the acupuncture group needled the SP6 point for 20 min after modeling; the moxibustion group stimulated the acupoint with mild moxibustion for same time. Record the contraction of the uterus by applying the BL-420F biological function experimental system; observe the OTR by RT-PCR in uterine of 7 rats in each group. Results： Compared with the saline group, all the intensity indicators of uterine contractions and the expressions of OTR mRNA in uterine were increased significantly in model group （P〈0.01 or P〈0.05）. Compared with model group, the contraction waves and the expression of OTR mRNA of the two treatment groups both significantly decreased （P〈0.01）, as well as the uterine activity of the moxibustion group （P〈0.05）. Furthermore, the expression lever of OTR mRNA in moxibustion group was obviously reduced than the acupuncture group （P〈O.O1）. Conclusion： Whether acupuncture or moxibustion at ＂＇Sanyinjiao＇ （SP6）＂ can improve the pathologic features of dysmenorrhea with cold syndrome, and the curative effect of moxibustion at ＂＇Sanyinjiao＇（SP6）＂ is better.

关 键 词：痛经 寒凝 针刺 艾灸 三阴交 OTR 大鼠 

分 类 号：R285.5[医药卫生—中药学]