滇西横断山区家畜体表蜱类调查及鉴定  

Tick species and genetic variants analysis of tick gene in Hengduan Mountains,west Yunnan Province,China

作　　者：亚红祥[1] 沈姝[2] 苏正元[2] 邓菲[2] 张云智[1] 

机构地区：[1]云南省地方病防治所/云南省自然疫源性疾病防控技术重点实验室,大理671000 [2]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国人兽共患病学报》2016年第10期865-870,共6页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.81260437;No.81060132);传染病预防控制国家重点实验室基金(No.2013SKLID302);科技基础性工作专项重点项目(No.2013FY113500)~~

摘　　要：目的调查滇西横断山区家畜体表蜱的种类。方法采集家畜体表寄生的蜱虫,经形态学鉴定后,用PCR法扩增蜱虫样本的16SrRNA、12SrRNA、COI、ITS2的基因片断,测序后进行序列分析。结果共采集成虫蜱样本1 874只,经形态学鉴定为1科、1属(扇头蜱属Rhipicephalus)、4种,其中微小扇头蜱(R.microplus)1 637只(占87.35%)),镰形扇头蜱(R.haemaphysaloides)218只(11.63%),短小扇头蜱(R.pumilio)11只(0.59%),血红扇头蜱(R.sanguineus)8只(0.43%)。样品分子鉴定结果与形态学鉴定结果一致。系统发育树分析显示,微小扇头蜱Y2 16SrRNA、12SrRNA、COI、ITS2的基因序列分别与来自印度(EU918188)、贵州(KC503259)、马来西亚(KM246873)、贵州(KC503274)的微小扇头蜱在同一分支上,而与以往云南发现的微小扇头蜱不在同一分支;镰形扇头蜱Y5的16SrRNA、12SrRNA和COI基因序列分别与来自泰国(KC170743)、台湾(DQ003005)和湖南(KM083593)的镰形扇头蜱在同一分支上;短小扇头蜱Y6和Y01的ITS2基因序列与来自澳大利亚的短小扇头蜱(AF271282)在同一分支上。结论滇西横断山区家畜体表蜱以微小扇头蜱为优势种,短小扇头蜱为云南境内首次发现。To investigate the species composition and genetic variants of ticks in Hengduan Mountains of the western Yunnan Province, 1 874 individual ticks were captured from the body surface of domestic animals and were identified as four species of one genus of one family by species morpholog. Of them, 97.35% （1 637/1 874） of ticks were Rhipicephalus microplus, 11.63% （218/1 874） Rhipicephalus haemaphysaloides, 0. 59% （11/1 874） Rhipicephalus pumilio and 0. 43% （8/1 874） Rhipicephalus sanguineus. The 16S rRNA, 12S rRN, COI and ITS2 segments of ticks were amplified separately by PCR and sequenced. Phylogenetic analysis showed that R. microplus Y2 （sample number） was in the same branch with R. microplus from Inida （EU918188）, Guizhou of China （KC503259）, Malaysia （KM246873） and Guizhou of China （KC503274） through 16S rRNA, 12S rRNA, CO1 and ITS2 sequences analysis respectively, but was different R. microplus fomerly reported from Yunnan of China. R. haemaphysaloides Y5 was in the same branch with R. haemaphysaloides from Thailand （KC170743）, Taiwan （DQ003005） and Hunan of China （KM083593） through 16s rRNA, 12S rRNA and COI sequences analysis respective- ly. R. pumilio Y6 and Y01 were in the same branch with R. pumilio from Australian （AF271282）. It was concluded that R. microplus is the dominant tick species of domestic animals in Hengduan Mountains, the west of Yunnan Province. In addition,R. pumilio may be a new record in Yunnan.

关 键 词：蜱 PCR检测 序列分析 云南 

分 类 号：R384.4[医药卫生—医学寄生虫学]