大肠杆菌O-抗原血清型鉴定研究进展  

Research progress on identification of Escherichia coli O-antigen serogroups

作　　者：刘璨颖 张济培[1] 王丙云[1] 

机构地区：[1]佛山科学技术学院,佛山528231

出　　处：《中国人兽共患病学报》2016年第10期928-933,共6页Chinese Journal of Zoonoses

基　　金：广东普通高校青年创新人才项目(No.2015KQNCX173)~~

摘　　要：大肠杆菌在一定条件下能引发宿主疾病,其表面O-抗原与毒力有关。O-抗原的化学组成和结构具有高度多样性,并且O-抗原血清型种类与大肠杆菌致病性有一定联系。因此,大肠杆菌O-抗原血清型鉴定对流行病学调查,防御和控制致病性大肠杆菌病有重要意义。传统的血清学分型方法耗时长、费用高、准确度不理想。随着科学技术的发展,研究者对大肠杆菌196种O-抗原基因簇进行了破译,比较分析了不同O-抗原基因簇序列,并针对基因簇中特异性DNA序列设计分子标记,运用PCR方法对O-抗原血清型进行分型,其中包括一般PCR、多重PCR、实时PCR、DNA芯片和微球悬浮列阵法。此外,还有rbf-限制性片段长度多态性分析法,磁性微球免疫分析法和基于全基因组序列预测法,这些方法丰富了O-抗原血清型鉴定方法,弥补了传统血清学分型方法的不足。本文概述了O-抗原合成基因簇序列和O-抗原血清型鉴定方法相关研究进展。Escherichia coli could cause diseases under certain conditions, O-antigens contribute to the virulence of E. coli. The chemical composition and structure of O-antigen on the surface of E. coli have high diversity, while specific O-antigen serotype have some connections with certain pathogenicity of E. coli. Thus, identification of O-antigen serotypes is important for epidemiological studies and control of diseases caused by pathogenic E. coli. The traditional serological typing method is time- consuming, expensive and inaccurate. With the development of science and technology, 196 O-antigen synthesis gene clusters of E. coli were sequenced, different O-antigen synthesis gene clusters were compared and analyzed, and molecular markers for specific DNA sequences were designed. PCR method was applied for serotyping O-antigens, including single PCR, multiple PCR, real-time PCR, DNA microarray and microbead-based suspension array. Besides, restriction of the amplified O-antigen gene cluster, mierobead-based immunoassay and whole genome sequencing were also used for serotyping O-antigens. These methods would enrich the identification method of E. coli O-antigen serogroups and make up the deficiency of traditional serological typing method. This review focuses on the research progress about O-antigen synthesis gene cluster and O-antigen serotype identification methods.

关 键 词：大肠杆菌 O-抗原 血清型分型 

分 类 号：Q939[生物学—微生物学]