雷藤舒(LLDT-8)对成纤维样滑膜细胞分泌趋化因子的影响  

Effect of (5R)-5-hydroxytriptolide(LLDT-8) on chemotactic factor in fibroblast-like synoviocytes

作　　者：童萍 刘佳 何东仪 

机构地区：[1]上海光华中西医结合医院关节内科,上海200052

出　　处：《现代免疫学》2016年第6期448-454,共7页Current Immunology

基　　金：国家自然科学基金面上项目(81273979);上海市中西医结合重点病种建设项目-类风湿关节炎(zxbz2012-05);上海市中医临床重点学科建设项目-中医痹病学(ZYXK2012012);上海市中医临床基地建设项目(ZY3-LCPT-1)

摘　　要：研究(5R)-5-羟基雷公藤内酯醇(雷藤舒,LLDT-8)对TNF-α联合IL-17诱导类风湿关节炎(rheumatoid arthritis,RA)成纤维样滑膜细胞(fibroblast-like synoviocytes,FLS)分泌趋化因子GRO-α、ENA-78、MCP-1、MIP-1α和RANTES的影响。ELISA法检测FLS上清液中GRO-α、ENA-78、MCP-1、MIP-1α和RANTES的浓度;RT-PCR法检测FLS中GRO-α、ENA-78、MCP-1、MIP-1α和RANTES mRNA的表达。结果显示LLDT-8可显著抑制TNF-α联合IL-17诱导的FLS上清液中GRO-α、ENA-78、MCP-1、MIP-1α和RANTES的表达;也可有效抑制TNF-α联合IL-17诱导的FLS GRO-α、ENA-78、MCP-1、MIP-1α和RANTES mRNA的表达。该研究提示LLDT-8通过抑制FLS产生GRO-α、ENA-78、MCP-1、MIP-1α和RANTES而在RA的治疗中发挥抗炎作用。We set out to explore the effect of LLDT-8 on the expression of chemotactic factor GRO-a, ENA-78, MCP-1, MIP- 1α,RANTES by TNF-α and IL-17 induced rheumatoid arthritis（RA） fibroblast-like synoviocytes（FLS）. The concentrations of GRO-α,ENA-78,MCP-1 ,MIP-1α,RANTES in FLS supernatant were examined by ELISA; the mRNA levels of GRO-α,ENA- 78,MCP-1,MIP-1α,RANTES in FLS were assessed by RT-PCR. We showed that LLDT-8 was effective to inhibit the secretion of GRO-α,ENA-78,MCP-1 ,MIP-1α,RANTES in FLS induced by TNF-α and IL-17. It was also effective to inhibit the ex- pression of GRO-α,ENA-78,MCP-1,MIP-la,RANTES mRNA in FLS. The results suggest that LLDT-8 has anti-inflammatory effect for rheumatoid arthritis by inhibiting the expression of GRO-α,ENA-78,MCP-1 ,MIP-1α,RANTES in FLS.

关 键 词：雷藤舒 类风湿关节炎 滑膜细胞 趋化因子 

分 类 号：R392.12[医药卫生—免疫学]