胶原诱导性关节炎小鼠Th17细胞及相关信号蛋白的动态变化  

Dynamic changes of Th17 cells and related signaling protein of collagen-induced arthritis mice

作　　者：王弥[1] 封桂英[1] 宋鸿儒[1] 邢恩鸿[2] 郭亚春[1] 安高[1] 赵晓菲[1] WANG Mi FENG Gui-Ying SONG Hong-Ru et al(Chengde Medical College, Chengde 067000, Hebei, Chin)

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院

出　　处：《中国老年学杂志》2016年第22期5501-5503,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(81273986)

摘　　要：目的研究鸡Ⅱ型胶原蛋白诱导性关节炎(CIA)小鼠淋巴细胞Th17及其转录激活因子STAT3和细胞因子信号蛋白抑制分子SOCS3的动态变化。方法取168只DBA1/J小鼠随机平均分为模型组和对照组,用制备的Ⅱ型胶原乳剂免疫模型组小鼠,在初次免疫后第7、14、21天及加强免疫后第7、14、35天在无菌条件下取腹股沟淋巴结,应用流式细胞术和Western印迹技术检测各组Th17细胞及STAT3、SOCS3的变化情况。结果模型组Th17细胞在加强免疫14 d时比正常组明显升高(P<0.05)。模型组STAT3的表达量在初次免疫14 d时比正常组显著升高(P<0.05);加强免疫14、21 d比正常组显著升高(P<0.05)。模型组SOCS3的表达量在初次免疫14 d时开始上升并持续到加强免疫14 d,与正常组比较具有明显差异(P<0.05)。结论 Th17细胞参与了RA的炎症进展,其主要在CIA小鼠病程进展中期起作用,Th17细胞在CIA小鼠病程中的变化与转录激活因子STAT3和细胞因子信号蛋白抑制分子SOCS3有关。Objective To study the dynamic changes of Thl7 ceils and STAT3,SOCS3 in lymphoglandula of chicken type II colla- gen-induced arthritis （CIA）mice and investigate the relationship between the development of rheumatoid arthritis and the changes of Thl7 cells and STAT＇3,SOCS3. Methods 168 DBA1/J mice were randomly divided into control group（n= 12）and CIA group（n= 12）. They were respectively picked the lymphoglandula under the sterile condition on the initial immunization 7 d, 14 d,21 d and 7 d, 14,21 d,35 d after booster immunization. Then the dynamic changes of Thl7 cells were detected by flow cytometry and STAT3 ,SOCS3 were detected by Western blot in different periods. Results Compared with the control group,the level of Thl7 in the model group was significantly increased on the 14th day after booster immunization （P0.05） ;The level of STAT3 in the model group was significantly higher than the control group on the 14th days after initial immunization （P〈0.05）. However, on the 14 days and 21 days after booster immunization, the level of STAT3 was in- creased obviously than the control group （ P〈0.05 ）. The level of SOCS3 was increased significantly on the 14th day after initial immunization （P〈0.05）, and still higher than the control group until to the 14 days after booster immunization （P〈0.05）. Conclusions The dynamic changes of CIM＋Th17 and STAT3 ,SOCS3 might be associated with the progress of RA.

关 键 词：胶原诱导的关节炎 TH17细胞 STAT3 SOCS3 

分 类 号：R593.22[医药卫生—内科学]