垂盆草人工种子制作技术研究  

Preparation Technique on Artificial Seeds of Sedum sarmentosum

作　　者：邢小姣 陆婷[1] 马楠[2] 雷江丽[2] 

机构地区：[1]新疆农业大学林学与园艺学院,新疆乌鲁木齐830052 [2]深圳市中国科学院仙湖植物园,广东深圳518000

出　　处：《西北林学院学报》2016年第6期169-174,共6页Journal of Northwest Forestry University

基　　金：深圳市城市管理局基金项目(2015023)

摘　　要：选用垂盆草2-4mm带腋芽茎段作为包埋外植体,研究海藻酸钠浓度、激素配比、活性炭、离子交换时间及人工种子培养条件对人工种子成型、萌发及成苗的影响,探索适合垂盆草的人工种子制作方法。结果表明,采用滴珠法,以MS＋4%海藻酸钠＋1.0mg·L^-1 6-BA＋0.1mg·L^-1GA3＋0.5mg·L^-1 NAA为人工胚乳,与2%CaCl2水溶液发生离子交换反应15min后获得的人工种子呈规则的球形、富弹性,在MS培养基中培养20d萌发率达95%,30d生根率达90%,在培养皿中催芽成苗率达60%,为制备垂盆草人工种子的适宜方法。Effects of synthetic endosperm components, activated carbon and germination conditions on the germination and rooting rate of artificial seeds of Sedum sarmentosum were researched by using 2-4 mm shoot cuttings with the buds used as propagules. The optimal medium used as artificial endosperm was MS ＋4% sodium alginate＋1. 0 mg · L^-1 6-BA＋0.1 mg· L^-1 GA3 ＋0.5 mg· L^-1 NAA. The spherical and elastic artificial seeds were prepared after dropping the shoot cuttings into the solution of 2% CaCI2 for 15 min. The germination rate of the artificial seeds was 95% after culture for 20 days in MS medium,the rooting rate was 90% after 30 days,and the seedling rate was 60% in a Petri dish germination. This formula was a suitable combination of artificial seeds for S. sarmentosum.

关 键 词：垂盆草 人工种子 人工胚乳 

分 类 号：S723.131[农业科学—林木遗传育种]