藏羚羊基因组微卫星分析与初步验证  

Genome-wide Characterization and Identification of Microsatellites in Pantholops hodgsoni

作　　者：都玉蓉[1,2] 谭春敏 徐永涛[1] 周智红[1] 马建滨[1,2] 郭松长[4] 

机构地区：[1]青海师范大学生命与地理科学学院,西宁810008 [2]青海省青藏高原药用动植物资源重点实验室,西宁810008 [3]青海省三江源民族中学,西宁810001 [4]中国科学院高原生物适应与进化重点实验室,西宁810007

出　　处：《四川动物》2016年第6期845-851,共7页Sichuan Journal of Zoology

基　　金：青海省应用基础研究计划项目(2013-Z-751;2013-Z-750)

摘　　要：为明确藏羚羊Pantholops hodgsoni核DNA中微卫星分布情况和特征,利用MISA工具对藏羚羊基因组进行微卫星扫描。在全长2 696.89 Mb的藏羚羊基因组中,搜索到723 135个微卫星座位,其中完全型微卫星有675 809个。6种重复类型中,单核苷酸重复最多,有471 142个,占69.72%;其次是二核苷酸、三核苷酸,分别为88 832个和86 658个,占13.14%和12.82%;六核苷酸最少,仅215个,约占0.03%。以藏羚羊基因组DNA为模板对微卫星座位进行验证,在100个微卫星座位中筛选到8个具有多态性的微卫星座位,多态比例约为8%。本研究将为研究藏羚羊微卫星标记、群体遗传多样性、藏羚羊保护生物学提供基础。To investigate the distribution and feature of simple sequence repeats or microsatellites （ 1 - 6 bp repeats）, we screened the entire genome of Tibetan antelope （Pantholops hodgsoni） （2 696.89 Mb） by using MISA. A total of 723 135 microsatellite loci including 675 809 perfect microsatellites were detected and characterized within the whole genome. Among these microsatellites, the most abundant repeat type was mononucleotide （69.72%）, followed by di- （13.14%）, tri- （ 12. 82% ）, penta- （ 2. 28% ）, tetra- （ 2. 01% ）, hexa- （ 0.03% ） nucleotide repeat types, and the motifs AC/GT （9.09%） and ACG/CTG （4. 64% ） appeared in high frequency. An approximate evaluation was conducted by randomly amplifying 100 microsatellite loci, and 8 loci （8%） were proved to be polymorphic. This study provide abundant microsat- ellite markers for analysis of genetic variation, construction of genetic map, as well as the conservation biology investigation in P. hodgsoni.

关 键 词：藏羚羊 基因组 微卫星 多态性 

分 类 号：Q953[生物学—动物学]