水稻多蘖矮杆突变体htd7(t)的遗传分析与基因定位  

Genetic Analysis and Gene Fine Mapping of A High-tillering and Dwarf Mutant htd7(t) in Rice(Oryza sativa L.)

作　　者：王军[1,2] 范方军[1,2] 朱金燕[1,2] 李文奇[1,2] 王芳权[1,2] 仲维功[1,2] 杨杰[1,2] 

机构地区：[1]江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心,南京210014 [2]扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009

出　　处：《中国农学通报》2016年第33期138-142,共5页Chinese Agricultural Science Bulletin

基　　金：公益性行业科研专项"粮食作物精确定量栽培技术研究与示范"(201303102);江苏省现代农业重点研发项目"抗穗颈瘟;褐飞虱优良食味粳稻新品种选育"(BE2015355);国家重大农技推广项目"洪泽县粮食作物新品种;新技术示范基地"(NG[15]003)

摘　　要：分蘖是水稻最重要的农艺性状之一,其决定水稻的最终产量。多蘖矮杆突变体htd7(t)是粳稻品种‘日本晴’经350 Gy的60Co-γ射线辐射处理后产生的突变体。为了克隆HTD7(t)基因,将htd7(t)与‘9311’配制正反杂交组合进行遗传分析发现,htd7(t)多蘖矮杆性状是受1对隐性核基因控制。利用SSR分子标记将HTD7(t)初步定位在第11染色体分子标记RM21与RM254之间,遗传距离分别为5.6 c M和3.2 c M。利用已经公布的水稻基因组数据,在该基因附近新发展了13对In Del标记,对HTD7(t)进行精细定位。根据定位结果构建覆盖HTD7(t)基因的BAC重叠群,最终将HTD7(t)定位在In Del11-3和In Del11-5之间的64.8 kb的物理距离内。Tillering is one of the most important agronomical characters of rice that determines the final yield.A rice mutant with a high-tillering capacity and dwarf, designated as htd7（t）, was isolated from the offspring ofthe japonica rice（Oryza sativa L. ssp. japonica） variety‘Nipponbare＇treated with 350 Gy60Co-γ radiation. Toclone the HTD7（t） locus, positive and negative cross combinations were developed between htd7（t） and‘9311＇,genetic analysis found that htd7（t） was controlled by one recessive nuclear gene. HTD7（t） was roughly mappedbetween two microsatellite markers RM21 and RM254 on the chromosome 11, with genetic distances of 5.6 c Mand 3.2 c M, respectively. According to the published rice genome data, 13 new In Del（Insertion- Deletion）markers were developed around HTD7（t）, and then fine mapping of HTD7（t） was conducted. The BAC contig of HTD7（t） was constructed based on the mapping results, and HTD7（t） was further mapped between two In Delmarkers, In Del11-3 and In Del11-5, with physical distance of 64.8 kb.

关 键 词：水稻 多蘖矮杆 基因定位 htd7(t) 

分 类 号：S330[农业科学—作物遗传育种]