维药异常黑胆质成熟剂对胰岛素抵抗大鼠的影响  

The effect of Abnormal Savda Muziq on insulin resistant rat model

作　　者：阿迪力.阿不都肉苏力 帕力万 夏依丁.哈力克 卡依沙尔 Denis Dubrovin 

机构地区：[1]新疆医科大学维吾尔医学院,乌鲁木齐830011 [2]新疆医科大学第一附属医院内分泌科,乌鲁木齐830054 [3]圣彼得堡巴甫洛夫国立医科大学,俄罗斯197000

出　　处：《新疆医科大学学报》2016年第12期1601-1605,1609,共6页Journal of Xinjiang Medical University

基　　金：国际合作项目(87E2A0313397)

摘　　要：目的探讨不同剂量的异常黑胆质成熟剂（ASM）对胰岛素抵抗（IR）SD大鼠的影响,研究ASM对胰岛素抵抗的预防和治疗作用。方法选用雄性SD大鼠80只,体质量（200±20）g,随机分为对照组、预防组（高、中、低剂量）、胰岛素抵抗组（模型建立后再分为模型组、治疗高、中、低剂量组,每组10只。预防组以高脂饲料饲养,同时不同剂量的ASM灌胃。治疗组模型建立后给予不同剂量的ASM分别灌胃。第十周检测血脂四项、空腹葡萄糖、空腹胰岛素水平、胰岛素敏感指数（ISI）、胰岛素抵抗指数（HOMA-IR）,结果与对照组和模型组比较,探讨模型是否建立和药物的预防作用。治疗组用ASM干预21 d后检测血脂四项、空腹葡萄糖、空腹胰岛素水平、胰岛素敏感指数（ISI）、胰岛素抵抗指数（HOMA-IR）,口服糖耐量（OGTT）,结果与对照组和模型组比较,探讨不同剂量的ASM对胰岛素抵抗的治疗作用。结果 （1）全程干预ASM后,预防3个不同剂量（PH、PM、PL）组的FBG均小于模型组和对照组,FINs值也小于模型组（P〈0.05~0.01）,HOMA-IR值同样下降（P〈0.01）。胰岛素抵抗模型建立后用AMS干预21 d,TH、TM和TL组的FINS值均小于模型组和对照组,TH组和TL组与模型组比较,FINs值差异有统计学意义（P〈0.05）。与模型组比较,TH、TM和TL组中HOMA-IR值明显下降（P〈0.05）,ISI值明显升高（P〈0.05）。（2）预防3个不同剂量（PH、PM、PL）组的HDL-C、LDL-C、TG值与模型组比较差异均有统计学意义（P〈0.05~0.01）,药物浓度越高越接近对照组。治疗3个不同剂量组的LDL和TG值小于模型组,TL组的TG值与模型组比较差异有统计学意义（P〈0.01）。（3）治疗3个不同剂量组30 min的血糖水平高于模型组和对照组（P〈0.05）,恢复正常水平比模型组快并接近于对照组。结论 ASM对大鼠胰岛素抵抗的产生有预防和治疗作用,能够改善胰岛Objective To study the therapeutic and preventive effect of Abnormal Savda Muziq（ ASM） on insulin resistant（ IR） rat model. Methods 80 male SD rats（ 180 ± 20 g） were randomly divided into control group,IR model group and ASM preventive groups（ low dose,middle dose,high dose）,and ASM treatment groups（ low dose,middle dose,high dose） respectively. Except the control group,other rats were fed with high fat diet to establish IR model. And the preventive group were fed with high fat diet plus orally administrated with low,middle,high dose of ASM respectively,10 weeks later testing results of blood lipid level,fasting blood glucose（ FBG）,fasting insulin（ FIN）,insulin sensitivity index（ ISI）,HOMA-IR was compared with IR model group and control group to investigate if the animal model established or not and evaluate the preventive role of ASM on metabolic syndrome; after the IR model was established,the treatment groups rats were orally administrated different doses of ASM for three weeks. Three weeks later,all the same indexes of the above were measured and compared with model and control group to investigate the therapeutic effect of ASM on insulin resistant. Results（ 1） After ASM intervention,the FBGs of the 3 doses of preventive groups were less than that in the model group and the control group,and the FINs values of them were less than that in the model group（ P 0. 05）,additionally,the HOMAIR value decreased（ P 0. 01）. After 21 d intervention of ASM on established insulin resistance model rats,the FINS values of the 3 doses of treatment groups were less than that in the model group and the control group,and there was significant difference between high dose group and low dose group（ P〈0. 05）. Compared with the model group,the HOMA-IR values of the 3 doses of treatment groups decreased significantly（ P〈0. 05）,while the ISI values of them were significantly increased（ P〈0. 05）.（ 2） Compared with the model group,the values of TG,LDL-C and HDL-C of the

关 键 词：胰岛素抵抗(IR) 异常黑胆质成熟剂(ASM) 血脂 

分 类 号：R29[医药卫生—民族医学]