低能量体外冲击波对兔膝骨关节炎软骨细胞修复能力和MAPK通路蛋白的影响  

The influence of Low energy extracorporeal shock wave to rabbit knee osteoarthritis cartilage repair ability and the MAPK pathway

作　　者：凌琳[1] 

机构地区：[1]广州医科大学附属第五医院康复医学科,广东广州511477

出　　处：《解剖学研究》2016年第5期380-383,390,共5页Anatomy Research

摘　　要：目的研究采用低能量体外冲击波冲击刺激对兔膝骨关节炎软骨细胞生长修复的影响,并探讨其是否通过调控上游细胞内MAPK通路蛋白ERK、JUK的表达而抑制MMPS的表达,从而减少细胞外基质的降解和降低炎症因子对软骨细胞的破坏作用。方法选取30只健康大新西兰兔分三组各10只。处理组与模型组采用hunt法切断兔膝关节内半月板后前交叉韧带以建立膝骨关节炎模型,对照组未给予如何处理;其中处理组采用0.16 Mpa/次,每侧1200的体外冲击波治疗,一周一次,余2组不采用任何处理措施,检测软骨细胞损伤和修复情况,并分析组织细胞内MMPs蛋白、关节滑液炎性因子IL-1β、TNF-α和MAPK通路蛋白ERK、JUK蛋白的表达。结果处理组兔膝关节软骨变薄、表面纤维增生呈修复反应,而模型组可见典型骨关节炎的膝关节改变;模型组Mankin评分均高于对照组和处理,差异具有统计学意义(P<0.05)。免疫组化显示处理组软骨MMP-1和MMP-13表达水平亦明显高于对照组,关节滑液炎性因子IL-1β、TNF-α亦明显升高,组间比较差异具有统计学意义(P<0.05)。western blot显示ERK、JUK蛋白在模型组中表达最高,处理组在治疗后其水平明显降低(P<0.05)。结论低能量体外冲击波刺激膝骨关节炎软骨细胞,可以降低关节MMP蛋白与炎性因子水平,从而促进兔膝关节软骨细胞的修复,其作用机制可能是通过调控上游细胞内MAPK通路蛋白ERK、JUK的表达而实现。Objective To study the influence of Low energy extracorporeal shock wave to rabbit knee osteoarthfitis cartilage repair ability, and to explore whether it can regulate the expression of MAPK pathway proteins of ERK and JUK protein that inhibits degradation of extracellular matrix and reduces inflammation factors effect on the destruction to chondrocyte. Methods 30 healthy New Zealand rabbits were selected and divided into three groups. Treatment group and model group was used to cut off the Anterior cru- ciate ligament of rabbit knee joint meniscus, while control group was not given any measures. The treatment group were given extracor- poreal shock wave treatment for 0.16 Mpa/time, each side 1200, and the other two groups do not use any treatment. Cartilage cell inju- ry, repair and the TNF-α, IL-1 and ERK／ JUK protein expression were analyzed. Results A typical change of knee joint osteoarthritis is seen in model group, and the cartilage tissue is thin and being hyperplasia, which the Mankin score is significant higher than the con- trol group and model （P 〈 0.05）. MMP-1, MMP-13 expression and IL-1β, TNF-α levels in treatment group were significantly higher than that of control group and model group （P 〈 0.05）. Western blot showed that ERK,JUK protein expressed in model group had high- est level, and decreased after the treatment in treatment group （P 〈 0.05）. Conclusion Low energy extracorporeal shock wave can stimulate osteoarthritis chondrocytes, and reduce inflammatory factor and MMPS protein levels. Thereby, rabbit articular cartilage cells can be repaired. Its mechanism may be through regulating the upstream MAPK pathway proteins of ERK and JUK protein expression.

关 键 词：膝骨关节炎 体外冲击波 MMP MAPK 

分 类 号：R684.3[医药卫生—骨科学]