一氧化氮介导VEGF对大鼠任意型皮瓣存活的影响  

Influence of nitric oxide on survival of random pattern skin flap by mediating VEGF in rat model

作　　者：华德军[1] 郝丽文[2] 神兴勤[1] 高学柱[1] 

机构地区：[1]滕州市中心人民医院手足外科,山东滕州277500 [2]山东省立医院手足外科,山东济南250021

出　　处：《中国临床研究》2016年第11期1552-1555,共4页Chinese Journal of Clinical Research

摘　　要：目的探讨一氧化氮(NO)介导血管内皮生长因子(VEGF)对任意型皮瓣存活的影响。方法按照随机数字表法将48只Wistar大鼠随机分为实验组(n=24)和对照组(n=24),48只大鼠均在其背部建立任意型皮瓣大鼠模型。实验组以左旋精氨酸作为NO供体,建模后即刻在大鼠腹腔内注射左旋精氨酸(300 mg/kg加2 ml/kg生理盐水),之后每隔24 h注射1次,直至第7天;对照组每次腹腔注射2 ml/kg生理盐水。分别应用SABC免疫组化法及HE染色检测VEGF蛋白的表达及新生血管增生情况,RT-PCR法检测VEGF mRNA的表达;应用皮瓣存活长度比皮瓣实际长度计算皮瓣存活率。结果与对照组比较,实验组胞浆染色为深棕色颗粒,分布密集,皮瓣组织血管内皮细胞内染色加深,棕色颗粒分布集中。实验组和对照组均有不同程度的微血管增生,实验组新生微血管增生更明显。建模后第1天两组皮瓣微血管计数差异无统计学意义(P>0.05);对照组建模后第7天(7.60±2.56)根/每高倍视野,实验组建模后第7天(12.82±4.17)根/每高倍视野,两组差异有统计学意义(P<0.01)。建模后第1天及第7天,实验组皮瓣组织VEGF mRNA相对表达水平均高于对照组[(0.88±0.07)vs(0.52±0.06),P<0.05;(1.74±0.12)vs(1.10±0.08),P<0.01]。术后第7天实验组大鼠皮瓣平均存活率为(74.81±5.36)%明显高于对照组的(50.10±4.17)%,差异有统计学意义(P<0.01)。结论 NO介导VEGF的升高可以改善皮瓣移植术后的微循环,对皮瓣移植具有保护作用。Objective To explore the influence of nitric oxide （NO） on the survival of the random patterns skin flap by mediating vascular endothelial growth factor（VEGF）. Methods A total of 48 Wistar rats were randomly divided into experimental group and control group according to random number table method （ n = 24 each）. The random pattern skin flap model was established on rat back. In experimental group, taking exogenous L-arginin as NO donator, intraperitoneal injection of L-arginin （ 300 mg/kg） and normal saline （ 2 ml/kg） was given immediately after modeling, and normal saline （2 m]/kg） was given in control group （once every 24 hours for 7 days in both groups）. Taking rat skin flap survival sample,SABC immunohistochemistry and HE staining were used to detect the expression of VEGF protein and microvascular hyperplasia situation of skin flap. Reverse transeription-polymerase chain reaction （RT-PCR） method was used to detect the expression of VEGF mRNA in skin flap tissues. The survival rate of skin flap was calculated by the ratio of survival length of the skin flap to the actual length of the skin flap. Results Compared with control group, cytoplasm staining was dense dark brown particles, and dyeing deepened in vascular endothelial cells of skin flap tissues with centralized brown particle distribution in experimental group. The microvascular hyperplasia of different degree was found in both groups, and neomi- erovascular hyperplasia in experimental group was more significant. There was no significant difference in microvascular count of skin flap （per high power field） at the first day after modeling between experimental group and control group（ P 〉 0.05 ） but was significant difference at the seventh day after modeling [ （ 12.82 ± 4.17 ） vs （7.60 ± 2.56）, P 〈 0.01 ]. Compared with control group, the relative expression levels of VEGF mRNA at 1- and 7-day after modeling in experimental group were all significantly higher than those in control group [ （ 0.88 ±

关 键 词：任意型皮瓣 皮瓣移植 皮瓣存活率 血管内皮生长因子 一氧化氮 左旋精氨酸 微循环 大鼠 

分 类 号：R628[医药卫生—整形外科]