罗丹明水杨酰腙-铜络合物探针在半胱氨酸检测中的应用  

Application of rhodamine salicylidene hydrazone-Cu^(2+) complex probe to the detection of cysteine

作　　者：高超颖[1] 张丽[1] 杨杨[1] 段莉梅[1] 乌兰格日乐[1] 

机构地区：[1]内蒙古民族大学化学化工学院,内蒙古通辽028000

出　　处：《石油化工》2016年第11期1375-1379,共5页Petrochemical Technology

基　　金：国家自然科学基金项目(21461018);内蒙古自治区自然科学基金项目(2014BS0205);内蒙古民族大学科学研究基金项目(NMDGP1403);通辽市与内蒙古民族大学科技合作项目(SXYB 2012040)

摘　　要：以罗丹明B和5-硝基水杨醛等为原料合成了罗丹明水杨酰腙(Rb NS),再将Rb NS与铜离子络合得到稳定的铜络合物Rb NS-Cu^(2+)。以Rb NS-Cu^(2+)为探针,通过紫外-可见吸收光谱法研究了探针对半胱氨酸(Cys)和S^(2-)的选择性、识别能力和检测限。实验结果显示,Rb NS-Cu^(2+)探针溶液中加入Cys或S^(2-)时,吸收强度明显降低,颜色由紫红色褪至无色。Rb NSCu^(2+)探针对Cys的识别具有较强的抗干扰能力。Rb NS-Cu^(2+)探针可选择性识别较高浓度的S^(2-)溶液,但对较低浓度的S^(2-)溶液则不灵敏。Rb NS-Cu^(2+)探针对Cys溶液有较高的选择性识别和响应能力,对Cys的实际检测限为1×10^(-5) mol/L。Rb NS-Cu^(2+)探针还具有可逆性,这对检测生物体系及食品中硫醇的浓度具有一定的意义。Rhodamine salicylidene hydrazone （ RbNS ） was synthesized by the condensation of rhodamine B hydrazide and 5-nitro-salicylaldehyde, and then RbNS and Cu^2＋ formed a stable copper complex RbNS-Cu^2＋ in ethanol. The complex was used as a probe for the identification of cysteine and S^2-. The selectivity, identification ability and detection limit of the complex to cysteine and S^2- were investigated by means of UV-vis. Upon the addition of cysteine or S^2- to the RbNS-Cu^2＋ solution, the magenta color faded to colorless and the absorbance intensity decreased rapidly. Moreover, it also showed strong anti-interference capability when cysteine was identified. The high concentration S^2- could be identified by RbNS-Cu^2＋, but it could not work for low concentrations S^2-. RbNS-Cu^2＋ displayed high selectivity and sensitive response to cysteine, and the detection limit of the system was around 1 × 10^-5 mol/L. The detection process is reversible, and is significant for the detection of cysteine in food and biological systems.

关 键 词：罗丹明水杨酰腙 铜离子络合物 比色分析 半胱氨酸 

分 类 号：TQ224.7[化学工程—有机化工]